Recombinant Human GOLGA6L22 Protein: Research Guide

Recombinant Human GOLGA6L22 Protein is a research-grade recombinant protein belonging to the Golgin family, produced for use in studies of Golgi apparatus structure, membrane trafficking, and related cellular processes.

Product Name

Recombinant Human GOLGA6L22 Protein

SKU

rp377480

Protein Family

Golgin family (GOLGA6 subfamily)

Species Origin

Human (Homo sapiens)

Product Type

Recombinant protein

Recommended Use

Research use only; not for therapeutic or diagnostic use

Biological Background of GOLGA6L22

GOLGA6L22 (Golgin A6 Family-Like 22) is a member of the Golgin superfamily, a diverse group of coiled-coil proteins predominantly localized at the Golgi apparatus. Golgins serve as structural scaffolds and tethering factors that maintain the characteristic stacked morphology of the Golgi and facilitate vesicle trafficking between Golgi cisternae and other cellular compartments.

The GOLGA6 subfamily represents a relatively recently expanded group of Golgin-related proteins in the human genome. Members of this subfamily are characterized by extended coiled-coil domains, which are structural motifs known to mediate protein–protein interactions critical for membrane tethering events. GOLGA6L22, as a family-like member, is presumed to participate in similar Golgi-associated functions, although its precise biological role remains an active area of investigation.

Research into Golgin family proteins has broad implications for understanding intracellular transport, Golgi ribbon formation, and the molecular basis of diseases linked to Golgi dysfunction, including certain congenital disorders of glycosylation and neurological conditions.

Protein Specifications and Quality

Recombinant Human GOLGA6L22 Protein (SKU: rp377480) is produced using recombinant expression technology to deliver a research-grade protein suitable for a range of in vitro applications. The product is manufactured under controlled conditions to ensure batch-to-batch consistency.

Key specification considerations for researchers:

• Recombinant origin: Expressed in a suitable recombinant host system to yield human-sequence GOLGA6L22 protein.
• Purity: Intended for research use; specific purity grade should be confirmed on the product datasheet provided with the lot.
• Formulation: Researchers should consult the accompanying Certificate of Analysis (CoA) for the precise buffer composition, concentration, and any additives present in the supplied formulation.
• Quality control: Each lot undergoes quality control testing; results are documented in the CoA supplied with the product.

For the most current and lot-specific technical data, always refer to the product datasheet and CoA available from Aladdin Scientific.

Handling, Storage, and Stability Guidelines

Proper handling and storage of recombinant proteins are essential to preserve activity and prevent degradation. The following general best practices apply to Recombinant Human GOLGA6L22 Protein; researchers should always cross-reference these with the specific instructions provided in the product datasheet and CoA.

Storage

• Upon receipt, briefly centrifuge the vial before opening to collect contents at the bottom.
• For short-term storage (up to 1 month), store at 4 °C if the protein will be used promptly.
• For long-term storage, aliquot the protein into single-use volumes and store at −20 °C or −80 °C to minimize freeze-thaw cycles.
• Avoid repeated freeze-thaw cycles, as these can reduce protein integrity and activity.

Handling

• Work on ice whenever possible to maintain protein stability during manipulation.
• Use low-protein-binding tubes and pipette tips to minimize adsorptive losses.
• If a carrier protein such as BSA is not already present in the formulation, consider adding it for diluted working solutions to prevent non-specific adsorption.

Reconstitution

• If the product is supplied in lyophilized form, reconstitute according to the datasheet instructions, typically using sterile ultrapure water or the specified buffer.
• Allow the reconstituted protein to equilibrate before use; gentle mixing by swirling is preferred over vortexing.

Research Applications

Recombinant Human GOLGA6L22 Protein is intended for research use only and may be applicable across a range of in vitro experimental platforms relevant to Golgi biology and intracellular trafficking research. Potential applications include:

• Protein–protein interaction studies: As a Golgin family member with predicted coiled-coil domains, GOLGA6L22 may be used as bait or prey in pull-down assays or yeast two-hybrid validation experiments to identify interaction partners involved in Golgi tethering or vesicle capture.
• Antibody development and validation: Recombinant GOLGA6L22 protein can serve as an antigen for the generation and specificity validation of antibodies targeting this protein.
• Biochemical characterization: The recombinant protein can be used for structural or biophysical studies, including circular dichroism, dynamic light scattering, or limited proteolysis experiments to probe domain structure.
• Cell-free assay systems: Can be applied in reconstitution assays to investigate the molecular mechanisms of Golgi membrane tethering or vesicle docking in vitro.
• ELISA and immunoassay standards: Can function as a reference standard in immunoassay development targeting GOLGA6L22.

Note: Specific validated applications and performance data should be confirmed in the product datasheet. Researchers are encouraged to optimize conditions for their specific experimental system.

Ordering and Support Information

Recombinant Human GOLGA6L22 Protein is available exclusively for research use from Aladdin Scientific.

SKU	Product Name	Intended Use
rp377480	Recombinant Human GOLGA6L22 Protein	Research use only

To place an order or obtain a quotation, contact your local Aladdin Scientific representative or visit the Aladdin Scientific website. For technical questions regarding this product, including recommendations on experimental design, buffer compatibility, or application optimization, please reach out to Aladdin Scientific technical support.

Documentation available:

• Product Datasheet (with lot-specific technical parameters)
• Certificate of Analysis (CoA)
• Safety Data Sheet (SDS)

All products are for research use only and are not intended for human or animal therapeutic or diagnostic applications.

Frequently asked questions

What is the recommended storage temperature for Recombinant Human GOLGA6L22 Protein?

For long-term storage, aliquot the protein and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles. For short-term use within one month, storage at 4 °C is acceptable. Always refer to the specific lot datasheet and CoA for precise storage instructions.

What are the primary research applications for Recombinant Human GOLGA6L22 Protein?

This protein is applicable for protein–protein interaction studies, antibody generation and validation, biochemical and biophysical characterization, cell-free reconstitution assays related to Golgi membrane tethering, and as an immunoassay reference standard. Researchers should confirm validated applications in the product datasheet.

How should I reconstitute Recombinant Human GOLGA6L22 Protein if it is supplied in lyophilized form?

Reconstitute the lyophilized protein using sterile ultrapure water or the buffer specified in the product datasheet. After reconstitution, allow the protein to equilibrate at the appropriate temperature and mix gently by swirling rather than vortexing. Avoid repeated freeze-thaw cycles of the reconstituted material.

Is Recombinant Human GOLGA6L22 Protein suitable for use in clinical or diagnostic applications?

No. Recombinant Human GOLGA6L22 Protein (SKU: rp377480) is intended strictly for research use only and is not approved or validated for therapeutic, diagnostic, or clinical applications in humans or animals.

How can I minimize protein loss due to adsorption during experiments?

Use low-protein-binding microcentrifuge tubes and pipette tips for all handling steps. When preparing diluted working solutions, consider supplementing with a carrier protein such as BSA if not already present in the formulation, to reduce non-specific adsorption to tube and tip surfaces.

References

1. Munro S. The golgin coiled-coil proteins of the Golgi apparatus. Cold Spring Harb Perspect Biol. 2011;3(6):a005256. doi:10.1101/cshperspect.a005256
2. Gillingham AK, Munro S. Finding the Golgi: An address but no postcode. Curr Opin Cell Biol. 2016;39:99-105. doi:10.1016/j.ceb.2016.02.013
3. Aladdin Scientific. Recombinant Human GOLGA6L22 Protein, SKU rp377480. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.