Recombinant Human GPATCH1 Protein: Research Guide & Protocol

Recombinant Human GPATCH1 Protein is a research-grade recombinant protein featuring the conserved G-patch domain, produced for use in studies of RNA processing, pre-mRNA splicing regulation, and DEAH-box RNA helicase activation.

Product Name

Recombinant Human GPATCH1 Protein

SKU

rp372858

Protein Family

G-patch domain-containing protein family

Primary Function

Cofactor for DEAH-box RNA helicases; involved in pre-mRNA splicing and RNA processing

Species Origin

Human (Homo sapiens)

Recommended Storage

Store at -80°C; avoid repeated freeze-thaw cycles

Intended Use

Research use only (RUO); not for diagnostic or therapeutic use

Background: GPATCH1 Gene and Protein Function

GPATCH1 (G-patch domain-containing protein 1) is a member of the G-patch domain protein family, characterized by a conserved glycine-rich motif of approximately 50 amino acids known as the G-patch. G-patch proteins are predominantly found in eukaryotes and are functionally associated with RNA metabolism, particularly pre-mRNA splicing and ribosome biogenesis.

GPATCH1 acts as a cofactor for DEAH-box RNA helicases, a class of ATP-dependent enzymes that remodel RNA-protein complexes during the splicing cycle. By stimulating helicase activity, GPATCH1 plays a regulatory role in the fidelity and efficiency of spliceosome-mediated intron removal. Dysregulation of splicing factors, including G-patch proteins, has been implicated in oncogenesis and various human diseases, making GPATCH1 a protein of interest in both basic RNA biology and translational research.

The recombinant form of Human GPATCH1 produced by Aladdin Scientific (SKU: rp372858) enables in vitro biochemical studies, interaction assays, and functional characterization without the complexity of endogenous protein purification from cellular lysates.

Reconstitution and Storage Protocol

Proper reconstitution and storage of Recombinant Human GPATCH1 Protein are critical to maintaining activity and preventing degradation. Follow the guidelines below to ensure optimal experimental outcomes.

• Reconstitution: Briefly centrifuge the vial before opening to collect the lyophilized material. Reconstitute the protein in sterile, high-quality water (e.g., Milli-Q or equivalent) or in a buffer appropriate for your downstream application (typically PBS or HEPES-based buffer, pH 7.2–7.4). Allow the protein to dissolve gently; avoid vigorous vortexing. Adding a carrier protein such as 0.1% BSA (bovine serum albumin) is recommended to stabilize the protein during storage and prevent adsorption to tube walls.
• Short-term storage: Aliquoted protein may be stored at 4°C for up to 1 week.
• Long-term storage: Store at -80°C in single-use aliquots to minimize freeze-thaw cycles. Repeated freeze-thaw cycles can lead to protein aggregation, loss of activity, and degradation.
• Do not store in frost-free freezers that undergo automated defrost cycles, as temperature fluctuations can compromise protein integrity.

Note: Always consult the product-specific datasheet provided with your shipment for lot-specific reconstitution volumes and buffer recommendations, as these may supersede general guidelines.

Experimental Applications

Recombinant Human GPATCH1 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–protein interaction studies: GPATCH1 is known to interact with DEAH-box RNA helicases. Co-immunoprecipitation (Co-IP), pull-down assays, and surface plasmon resonance (SPR) can be used to map interaction interfaces and measure binding affinities.
• Helicase stimulation assays: Recombinant GPATCH1 can be used in coupled ATPase or helicase unwinding assays to functionally characterize its stimulatory effect on target helicases, providing mechanistic insight into spliceosome remodeling.
• Western blotting (WB) and ELISA: As a pure recombinant antigen, GPATCH1 protein can serve as a positive control or standard in immunoassays designed to detect endogenous GPATCH1 expression levels across cell lines or tissue samples.
• Structural studies: Purified recombinant protein may be used for X-ray crystallography, cryo-EM, or NMR studies aimed at resolving the three-dimensional structure of the G-patch domain and its complexes.
• Antibody validation: Recombinant GPATCH1 serves as a reliable antigen for validating anti-GPATCH1 antibody specificity and sensitivity.

Always optimize protein concentrations and buffer conditions empirically for each specific assay format.

Quality, Safety, and Regulatory Considerations

Research Use Only: Recombinant Human GPATCH1 Protein (rp372858) is intended exclusively for in vitro research purposes. It is not approved for use in diagnostic procedures, therapeutic applications, or any in vivo administration in humans or animals.

Purity and Quality Control: Aladdin Scientific's recombinant proteins undergo quality control testing prior to release. Users should refer to the Certificate of Analysis (CoA) included with each lot for purity data (typically assessed by SDS-PAGE), endotoxin levels, and activity data where applicable. If a CoA is not included in your shipment, contact Aladdin Scientific customer service to obtain the relevant documentation.

Safety: While recombinant GPATCH1 is not classified as a biohazardous material, standard good laboratory practice (GLP) should be observed. Wear appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection. Refer to the Safety Data Sheet (SDS) for handling, disposal, and emergency procedures.

Regulatory Compliance: Export of this product may be subject to applicable national and international regulations. Procurement teams should verify compliance requirements before ordering for international shipment.

Frequently asked questions

What is the recommended reconstitution buffer for Recombinant Human GPATCH1 Protein?

We recommend reconstituting the lyophilized protein in sterile water or a physiological buffer such as PBS or HEPES (pH 7.2–7.4). Adding 0.1% BSA as a carrier protein helps stabilize the protein and prevents loss due to adsorption. Always refer to the lot-specific CoA for the recommended reconstitution volume.

How should I store Recombinant Human GPATCH1 Protein to preserve its activity?

For long-term storage, aliquot the reconstituted protein and store at -80°C. Avoid repeated freeze-thaw cycles, as these cause aggregation and activity loss. Short-term storage at 4°C (up to one week) is acceptable for aliquots in active use. Do not store in frost-free freezers subject to automated defrost cycles.

What experimental applications is Recombinant Human GPATCH1 Protein validated for?

Recombinant Human GPATCH1 Protein is suitable for protein–protein interaction assays (Co-IP, pull-down, SPR), in vitro helicase stimulation assays, Western blot positive controls, ELISA standards, antibody validation, and structural biology studies. Optimization of conditions for each specific application is recommended.

Is Recombinant Human GPATCH1 Protein suitable for in vivo or diagnostic use?

No. This product is strictly for research use only (RUO). It is not intended for diagnostic, therapeutic, or any in vivo application in humans or animals. Researchers must comply with all applicable institutional and regulatory requirements when using this reagent.

How can I obtain a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for this product?

The Certificate of Analysis is typically included with your shipment. If it is missing, or if you require the Safety Data Sheet, please contact Aladdin Scientific customer service with your order number and lot number. Both documents are essential for proper use and compliance documentation.

References

1. Bohnsack MT, Sloan KE. The mitotic spindle checkpoint: diverse roles and novel targets for G-patch domain proteins. Biochemical Society Transactions. General reference for G-patch domain protein biology.
2. Wen X, et al. G-patch proteins: a new class of spliceosomal regulators involved in RNA helicase activation. General textbook-level reference; specific article details require verification.
3. Aladdin Scientific Product Page: Recombinant Human GPATCH1 Protein, SKU rp372858. Aladdin Scientific internal product documentation.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.