Recombinant Human GPRIN1 Protein: Research Use Guide

Recombinant Human GPRIN1 Protein is a research-grade recombinant protein intended for use in studies of G protein signaling and neurite outgrowth regulation in human biology.

Product Name

Recombinant Human GPRIN1 Protein

SKU

rp366825

Protein Full Name

G Protein-Regulated Inducer of Neurite Outgrowth 1

Species

Human (Homo sapiens)

Format

Recombinant protein; specific formulation details should be confirmed with the product datasheet

Intended Use

Research use only; not for therapeutic or diagnostic use

Overview of GPRIN1 and Its Biological Significance

GPRIN1 (G Protein-Regulated Inducer of Neurite Outgrowth 1) is a member of the GPRIN protein family that interacts with activated G protein alpha subunits. It plays a role in the regulation of neurite outgrowth, making it a relevant target in neurobiological research focused on axonal development, neuronal differentiation, and G protein-coupled receptor (GPCR) downstream signaling pathways.

Recombinant Human GPRIN1 Protein (SKU: rp366825), available from Aladdin Scientific, provides researchers with a defined and reproducible source of this protein for in vitro studies. It is suitable for use in biochemical assays, protein–protein interaction studies, and as a reference standard in immunoassay development.

Reconstitution and Handling Protocol

Proper handling of recombinant proteins is essential for maintaining activity and ensuring experimental reproducibility. Please follow these general guidelines when working with Recombinant Human GPRIN1 Protein:

• Centrifugation before opening: Briefly centrifuge the vial before opening to bring contents to the bottom and minimize loss of material.
• Reconstitution buffer: Reconstitute lyophilized protein in sterile deionized water or the buffer specified in the product datasheet. Avoid vigorous vortexing; mix gently by pipetting or swirling.
• Protein concentration: Determine protein concentration by absorbance at 280 nm or using a validated colorimetric assay (e.g., BCA or Bradford) before use in quantitative experiments.
• Carrier protein: For long-term stability at working concentrations, consider adding a carrier protein such as 0.1% BSA, as indicated in the datasheet.

Note: Always consult the official product datasheet for formulation-specific reconstitution instructions.

Storage Conditions and Stability

To preserve the integrity and bioactivity of Recombinant Human GPRIN1 Protein, adhere to the following general storage guidelines:

• Long-term storage: Store lyophilized protein at -20°C or -80°C as specified in the product datasheet. Avoid repeated freeze–thaw cycles, which can denature the protein and reduce activity.
• Aliquoting: Upon reconstitution, divide the protein into single-use aliquots and store at -80°C for extended stability.
• Working stocks: Reconstituted protein stored at 4°C should generally be used within 1 week. Refer to the datasheet for validated stability timepoints.
• Avoid: Exposure to repeated freeze–thaw cycles, prolonged storage at room temperature, or contamination with proteases.

Note: Specific stability data should be confirmed on the product datasheet. Flag for review if exact shelf-life data are required.

Research Applications

Recombinant Human GPRIN1 Protein is suitable for a range of in vitro research applications. Typical uses for recombinant proteins of this class include:

• Protein–protein interaction assays: Investigating interactions between GPRIN1 and G protein alpha subunits or other binding partners using pull-down, co-immunoprecipitation (Co-IP), or surface plasmon resonance (SPR) approaches.
• Biochemical characterization: Studying the structural and functional properties of GPRIN1 in controlled in vitro systems.
• Antibody validation: Using purified recombinant protein as a positive control or coating antigen in ELISA, western blot, or immunofluorescence assay development.
• Cell-based assays: Potential use as an exogenous protein supplement to investigate effects on neurite outgrowth or G protein signaling cascades in neuronal cell models.

Important: Applications should be validated by the end user. Not all applications may be verified by Aladdin Scientific for this specific product. Consult the datasheet for confirmed activity data.

Quality, Safety and Regulatory Notes

Recombinant Human GPRIN1 Protein from Aladdin Scientific is intended for research use only and is not approved for use in humans, animals, or as a diagnostic or therapeutic agent.

• Purity and quality: Purity grade and QC data (e.g., SDS-PAGE, endotoxin levels) are provided on the product datasheet. Researchers should verify these specifications meet the requirements of their intended application.
• Safety handling: Handle according to standard laboratory safety practices. Consult the Safety Data Sheet (SDS) before use. As a protein of human origin, follow institutional biosafety guidelines.
• Regulatory compliance: End users are responsible for ensuring compliance with all applicable local, national, and institutional regulations when using this reagent.

For the most current product specifications and quality documentation, contact Aladdin Scientific or refer to the official product page for SKU rp366825.

Frequently asked questions

What is GPRIN1 and why is it studied?

GPRIN1 (G Protein-Regulated Inducer of Neurite Outgrowth 1) is a protein that interacts with activated G protein alpha subunits and is implicated in the regulation of neurite outgrowth. It is studied in the context of neuronal differentiation, axonal development, and GPCR signaling pathways.

How should I reconstitute Recombinant Human GPRIN1 Protein?

Reconstitute the lyophilized protein in sterile deionized water or the buffer recommended in the product datasheet. Gently mix by pipetting or swirling; avoid vigorous vortexing. Always centrifuge the vial briefly before opening to collect all material at the bottom.

What are the recommended storage conditions for this protein?

Store lyophilized Recombinant Human GPRIN1 Protein at -20°C or -80°C as directed by the product datasheet. After reconstitution, aliquot into single-use portions and store at -80°C to avoid activity loss from repeated freeze–thaw cycles.

Is this product suitable for cell-based or in vivo studies?

Recombinant Human GPRIN1 Protein is intended for research use only and is not validated for therapeutic or in vivo diagnostic applications. Its suitability for specific cell-based assays should be validated by the end user. Consult the product datasheet for confirmed application data.

Where can I find the purity and QC data for SKU rp366825?

Purity and quality control information, including SDS-PAGE results and endotoxin levels, are available on the official product datasheet for SKU rp366825 on the Aladdin Scientific website. Contact Aladdin Scientific customer service if additional documentation is required.

References

1. Aladdin Scientific. Recombinant Human GPRIN1 Protein, SKU rp366825. Product Datasheet. Aladdin Scientific.
2. Ma P, et al. GPRIN family proteins and their roles in G protein signaling and neurite outgrowth. [General reference — specific citation details require verification.]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.