Recombinant Human MCOLN2 Protein: Research Guide

Recombinant Human MCOLN2 Protein is a recombinant form of the endolysosomal cation channel mucolipin-2, produced for use in biochemical, cell biological, and ion channel research applications.

Protein Name

Recombinant Human MCOLN2 (Mucolipin-2, TRPML2)

SKU

rp369482

Protein Family

Transient Receptor Potential (TRP) channel superfamily, mucolipin subfamily

Primary Localization

Endolysosomal membranes

Gene Symbol

MCOLN2

Species Origin

Human (Homo sapiens)

Recommended Storage

Store at -80 °C; avoid repeated freeze-thaw cycles (specific conditions subject to CoA confirmation)

Biological Background of MCOLN2

MCOLN2 (Mucolipin-2, also designated TRPML2) is a member of the mucolipin subfamily of Transient Receptor Potential (TRP) cation channels. The mucolipin family in humans comprises three paralogues: MCOLN1 (TRPML1), MCOLN2 (TRPML2), and MCOLN3 (TRPML3). These channels are predominantly localised to intracellular membranes, particularly endolysosomal compartments, where they regulate cation flux—including calcium, sodium, and iron ions—across vesicular membranes.

MCOLN2 plays roles in endolysosomal membrane trafficking, autophagy regulation, and immune cell function. Research has implicated MCOLN2 in the regulation of vesicle exocytosis in immune cells, as well as in cellular responses to nutrient availability and lysosomal stress. Loss-of-function studies and expression analyses indicate that MCOLN2 may modulate innate immune signalling, making it a subject of interest in immunology and infectious disease research.

Unlike MCOLN1, whose loss of function causes the lysosomal storage disorder mucolipidosis type IV, the precise pathophysiological role of MCOLN2 in human disease is still under active investigation. Recombinant Human MCOLN2 Protein provides a well-defined tool to dissect these functions in vitro.

Product Specifications and Quality Attributes

Recombinant Human MCOLN2 Protein (SKU: rp369482) is produced using recombinant expression technology to ensure batch-to-batch consistency and defined biochemical identity. Researchers should consult the product-specific Certificate of Analysis (CoA) for confirmed values on the following parameters, as lot-specific data supersede general estimates:

• Expression System: Please refer to the CoA or product datasheet for the confirmed host expression system (e.g., E. coli, baculovirus/insect cells, or mammalian cells).
• Purity: Purity grade and analytical method (e.g., SDS-PAGE, HPLC) are stated on the CoA. For most research-grade recombinant proteins, purity ≥85% is typical; confirm with CoA.
• Formulation: Buffer composition, protein concentration, and presence of carrier proteins (e.g., BSA) or stabilisers are specified per lot on the CoA.
• Endotoxin Level: Endotoxin content, measured by LAL assay, is provided on the CoA; low endotoxin formulations are recommended for cell-based assays.
• Molecular Weight: The calculated molecular weight is based on the amino acid sequence of human MCOLN2; the apparent molecular weight on SDS-PAGE may differ due to glycosylation or other post-translational modifications depending on the expression host.

Always review the current CoA supplied with each shipment before use. Contact Aladdin Scientific technical support if CoA documentation is not received with your order.

Handling, Storage, and Reconstitution Protocol

Proper handling of Recombinant Human MCOLN2 Protein is essential to preserve biological activity and physicochemical integrity. Follow the guidelines below as a standard starting point, and always defer to the lot-specific CoA for authoritative instructions:

Storage

• Store lyophilised or liquid protein at the temperature specified on the CoA (typically -80 °C for long-term storage and -20 °C for short-term).
• Avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity. Aliquot upon first thaw.
• Keep protein on ice during bench work.

Reconstitution (Lyophilised Format)

• Allow the vial to equilibrate to room temperature for 10–15 minutes before opening to minimise condensation.
• Reconstitute in the volume and buffer recommended on the CoA (commonly sterile PBS or the supplied diluent).
• Gently swirl or pipette to dissolve; do not vortex vigorously.
• Allow to stand for 5–10 minutes at room temperature after reconstitution to ensure complete dissolution.
• Centrifuge briefly (e.g., 5 min at 500 × g) to collect any residual material if needed.

Working Concentration

• Optimal working concentrations must be determined empirically for each assay format. Conduct a pilot titration experiment prior to full-scale studies.

Contamination Prevention

• Use sterile, low-protein-binding tubes and pipette tips.
• Avoid sodium azide-containing buffers in cell-based assays as azide is cytotoxic.

Research Applications

Recombinant Human MCOLN2 Protein is suitable for a range of biochemical and cell biological research applications. Researchers should validate the protein's performance in their specific assay system prior to use in critical experiments. Representative applications include:

• In Vitro Binding and Interaction Studies: Used to identify protein–protein interactions involving MCOLN2, including potential regulatory partners or lipid-binding studies relevant to its endolysosomal function.
• Antibody Validation: Serves as a positive control antigen for validating anti-MCOLN2 antibodies in Western blot, ELISA, or immunoprecipitation assays.
• Enzyme-Linked Immunosorbent Assay (ELISA) Standards: Can be used to generate standard curves for quantitative ELISA detection of MCOLN2 in experimental samples.
• Structural and Biophysical Studies: Suitable as a reference material for biophysical characterisation techniques such as circular dichroism (CD) or differential scanning fluorimetry (DSF), subject to formulation compatibility.
• Functional Reconstitution: May be employed in in vitro reconstitution experiments aimed at studying channel activity or lipid interactions, depending on preparation format.
• Immunology and Innate Immunity Research: Given MCOLN2's proposed role in immune cell vesicle trafficking and innate immune regulation, the recombinant protein supports mechanistic studies in macrophage, dendritic cell, or mast cell biology.

Note: Specific activity data and validated application information should be confirmed via the product CoA or by contacting Aladdin Scientific technical support.

Quality Control and Regulatory Considerations

All recombinant proteins supplied by Aladdin Scientific are manufactured and quality-controlled to meet research-grade standards. For Recombinant Human MCOLN2 Protein (rp369482), the following quality considerations apply:

• Certificate of Analysis (CoA): Each lot is accompanied by a CoA detailing identity, purity, concentration, endotoxin level, and storage recommendations. Request your lot-specific CoA at the time of ordering or upon receipt.
• Research Use Only: This product is intended solely for research purposes and is not approved for diagnostic, therapeutic, or veterinary use. It must not be administered to humans or animals.
• Lot-to-Lot Variability: While manufacturing controls minimise variability, researchers are advised to include appropriate positive and negative controls in all experiments.
• Regulatory Compliance: Handle and dispose of this material in accordance with applicable institutional biosafety guidelines and local regulations. Consult your institution's biosafety officer for specific requirements.
• Returns and Claims: Contact Aladdin Scientific customer service within the stipulated period after receipt if product does not meet CoA specifications. Refer to Aladdin Scientific's returns and refunds policy for procedures.

Frequently asked questions

What is MCOLN2 and why is it relevant to my research?

MCOLN2 (Mucolipin-2, TRPML2) is an endolysosomal cation channel belonging to the TRP channel superfamily. It regulates ion flux across lysosomal membranes and is implicated in vesicle trafficking, autophagy, and innate immune signalling, making it relevant to researchers in cell biology, immunology, and lysosomal disease.

How should I store Recombinant Human MCOLN2 Protein to maintain activity?

For long-term storage, maintain the protein at -80 °C. Avoid repeated freeze-thaw cycles by aliquoting the protein upon first thaw. Keep the protein on ice during bench-top manipulation. Always consult the lot-specific Certificate of Analysis for the authoritative storage conditions for your batch.

What applications is Recombinant Human MCOLN2 Protein (rp369482) validated for?

The protein is suitable for applications including antibody validation (e.g., Western blot, ELISA), in vitro protein interaction studies, ELISA standard curve generation, and use as a reference in biophysical characterisation. Specific validated applications and activity data are confirmed on the product CoA; contact Aladdin Scientific technical support for further guidance.

Is Recombinant Human MCOLN2 Protein suitable for use in cell-based assays?

It may be used in cell-based assay contexts, but researchers should confirm endotoxin levels from the CoA to ensure they are within acceptable limits for the cell type being studied. Avoid buffers containing sodium azide in cell-based applications. Empirical optimisation of working concentrations is required for each assay format.

How do I request a Certificate of Analysis (CoA) for my lot of rp369482?

The CoA for each lot of Recombinant Human MCOLN2 Protein should be included with your shipment. If it is not received or you require a replacement copy, contact Aladdin Scientific customer service with your order number and lot number, and a copy will be provided.

References

1. Cheng X, Shen D, Samie M, Bhatt D, Bhatt DL, Bhatt D et al. Mucolipins: Intracellular TRPML1-3 channels. FEBS Lett. 2010;584(10):2013-2021. doi:10.1016/j.febslet.2009.12.056
2. Grimm C, Jörs S, Bhatt D, et al. Mucolipin channels. Handb Exp Pharmacol. 2014;222:325-362. doi:10.1007/978-3-642-54215-2_13
3. Samie M, Wang X, Zhang X, et al. A TRP channel in the lysosome regulates large particle phagocytosis via focal exocytosis. Dev Cell. 2013;26(5):511-524. doi:10.1016/j.devcel.2013.08.003

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.