What is IPTG?· IPTG (isopropyl-β-D-thiogalactopyranoside) is a synthetic, non-metabolizable analog of galactose that binds the lac repressor and lifts repression to induce β-galactosidase or any gene downstream of a lac-operator promoter in E. coli · Because it cannot be cleaved by β-galactosidase, its cellular concentration remains constant throughout induction, offering reproducible control over gene expression Mechanism of Action· IPTG binds LacI repressor, triggering a conformational change ...

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Blue-white colony screening

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What is IPTG?

Updated Nov 12, 2026·3 min read

What is IPTG?

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