Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The TUNEL assay is one of the most commonly used methods for detecting DNA fragmentation in apoptosis studies. Its detection target is the 3'-OH terminus of fragmented DNA rather than the apoptotic pathway itself.
The DNA of apoptotic cells is cut into fragments of about 200 base pairs under the action of caspase-activated nuclease (CAD), forming DNA ladders. By detecting DNA fragmentation during apoptosis, the degree of apoptosis can be evaluated.
Updated Jun 2025·35 min read
Related topics
Techniques often explored alongside immunological experiments.
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