Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The blue color of nickel agarose beads arises from coordination between Ni²⁺ and immobilized ligands (e.g., NTA/IDA/TED-type chelators). When loading His-tagged proteins, the local coordination environment changes and the color often becomes temporarily lighter; if strong chelators such as ...
Updated Nov 2025·3 min read
Related topics
Techniques often explored alongside immunological experiments.
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