Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
In mammalian stable cell line generation, lentiviral/plasmid transduction selection, CRISPR functional screening, and multi-gene co-expression experiments, antibiotic selection systems are used to enrich cells that have successfully integrated or expressed resistance genes.
Hygromycin B is an aminoglycoside antibiotic. Enriched with highly polar functional groups such as hydroxyl and amino groups, it exhibits good water solubility and strong solvation capacity, and can interact with key structural domains of the ribosomal translation machinery, thereby inhibiting ...
Updated Jan 2026·2 min read
Related topics
Techniques often explored alongside immunological experiments.
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