Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The endoplasmic reticulum maintains protein homeostasis through molecular chaperones, endoplasmic reticulum-associated degradation, and endoplasmic reticulum-selective autophagy. Abnormal proteins that can be unfolded are mainly retrotranslocated through ERAD and degraded by the proteasome, ...
The proteolytic enzyme network in cell injury involves calpains, caspases, cathepsins, MMP/ADAM family proteases, and the ubiquitin-proteasome system. Different proteolytic enzymes correspond to Ca²⁺ overload, apoptotic execution, lysosomal injury, extracellular matrix remodeling, and ...
Ubiquitination is a highly complex type of post-translational modification. Through the coordinated action of ubiquitin molecules, ubiquitin chain structures, the E1-E2-E3 enzymatic cascade, and deubiquitinating enzymes, this system regulates substrate protein stability, subcellular ...
Updated May 202612 min read✓ Reviewed
Related topics
Techniques often explored alongside immunological experiments.
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