1,700+ technical articles by Aladdin Scientific's scientists on reagent selection, reaction mechanisms, assay design and formulation. New every week.
Poly(ethylene glycol) (PEG) is a widely used hydrophilic polymer that is commonly employed for the covalent modification of proteins, peptides and small-molecule drugs. By introducing PEG chains onto drug molecules or the surfaces of delivery carriers (PEGylation), their physicochemical ...
Poly(amidoamine) (PAMAM) dendrimers are a class of highly branched macromolecules featuring well-defined generations and precisely engineered three-dimensional architectures. Their molecular size, internal structural units, and terminal functional groups can all be finely tuned. Owing to these ...
Cell proliferation is one of the fundamental processes underlying organismal growth, development, reproduction, and heredity, and it is also a core step in cell fate regulation and the onset and progression of diseases. In in-vitro culture systems or at the tissue level, accurately evaluating ...
In studies of protein structure and function, antibody drug development, and enzyme engineering/production, the core of purification process design is to “select appropriate separation mechanisms based on the physicochemical properties of the protein, and rationally combine multiple ...
In real-time PCR, molecular hybridization, SNP genotyping and other nucleic acid assays, we typically rely on fluorescence signals to determine whether a target is present and at what abundance. However, if the system contains only a fluorescent dye and no “counterpart”, it becomes difficult ...
Accurately knowing protein concentration underpins downstream work such as enzymatic assays, quantitative Western blots, MS loading, and immunoassays. Because methods differ in principle, sensitivity, interference tolerance, and instrumentation needs, it’s useful to review 11 common options to ...
Eluting biotinylated proteins and DNA from streptavidin beads usually requires relatively harsh conditions, such as enzymatic digestion, extreme pH, organic solvents, or heating in the presence of excess biotin. The most appropriate elution strategy will depend on the properties of your ...
Streptavidin is a bacterial protein that binds the small molecule biotin with exceptionally high affinity. The streptavidin–biotin pair represents one of the strongest noncovalent interactions known. Streptavidin agarose beads take advantage of this interaction as a solid-phase platform to ...
Glutathione is a tripeptide antioxidant naturally produced in the body, and it plays a crucial role in safeguarding our proteins and DNA from damage caused by free radicals and oxidative stress. Beyond its physiological functions, this highly versatile molecule—and the proteins that interact ...