One of the biological activities of TNF is its ability to directly kill tumor cells.TNF binds to the corresponding receptor and then moves into the cell, where it is taken up by the target cell lysosomes leading to a decrease in lysosomal stability and the leakage of a variety of enzymes, causing cell lysis. There are great differences in the sensitivity of tumor cell lines to TNF-α. Treatment of tumor cells with actinomycin D, mitomycin C, and actinomycin ketone can significantly enhance TNF-α tumor cell killing activity.
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