FRK Antibody - Primärer Antikörper, specific to FRK

Cat. No.: Ab104145

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human FRK Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

FRK Antibody - Primärer Antikörper, specific to FRK

Synonyme

Beta cell Src homology tyrosine kinase antibody | BSK antibody | BSK/IYK antibody | C85044 antibody | Frk antibody | Frk fyn related kinaseantibody | FRK_HUMAN antibody | FYN related kinase antibody | FYN-related kinase antibody | GASK antibody | Gastroin

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Biochemische und physiologische Mechanismen

Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor.

Wirtsspezies

Kaninchen

Spezifität

FRK

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FRK.

Positive Kontrolle

WB: K562, MOLT-4 and Hep G2 cell lysates. IHC: Human breast cancer tissue. IF/ICC: K562 cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Immunogen affinity purified

Konzentration

1.0 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: FRK Antibody (Ab104145)

FRK Antibody (Ab104145) - Western Blot
All lanes: FRK Antibody (Ab104145) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 48.1 s

IF/ICC: FRK Antibody (Ab104145)

FRK Antibody (Ab104145) - IF/ICC
Immunofluorescent analysis of FRK staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with FRK Antibody (Ab104145) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IHC: FRK Antibody (Ab104145)

FRK Antibody (Ab104145) - IHC
Immunohistochemical analysis of FRK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FRK Antibody (Ab104145) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anwendung

Anwendung

Dilution info

IF/ICC

1/100-1/500

WB

1/500-1/1000

IHC

1/100-1/200

Zugehörige Ziele (menschlich)

FRK Tchem Tyrosine-protein kinase FRK (0 Activities)

Discover Target: FRK

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (FRK Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.