Gel Loading Buffer - for NA electrophoresis

Cat. No.: G485785

GRADE & PURITY for NA electrophoresis

Synonyms

6X DNA loading buffer | 6X DNA loading dye | 6X DNA loading solution | agarose gel loading buffer | agarose gel loading dye | agarose gel loading solution | sample loading buffer | sample loading dye | sample loading solution

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Gel loading buffer is used as a tracking dye during electrophoresis. The dye has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample before loading.Gel Loading Buffer has been used for loading polymerase chain reaction (PCR) amplified intergenic spacer region (ISR) sequence samples, PCR amplified DNA samples of the intestinal mucosa,Trypanosoma spDNA on agarose gel for electrophoresis.It is suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.

Specifications

Synonyms

6X DNA loading buffer | 6X DNA loading dye | 6X DNA loading solution | agarose gel loading buffer | agarose gel loading dye | agarose gel loading solution | sample loading buffer | sample loading dye | sample loading solution

Specifications & Purity

for NA electrophoresis

Biochemical and Physiological Mechanisms

Gel loading buffer is especially formulated for non-denaturing polyacrylamide and agarose gel electrophoresis of nucleic acids. The gel loading solution contains a bromophenol blue tracking dye and sucrose to add density and facilitate sample loading. EDT

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Note

If crystals form, dissolve them by heating the solution to 65C for 10 minutes.

Chemical and Physical Properties

• Solubility: Soluble in water at 20 °C