General Broth Medium is a fundamental core consumable for enrichment culture, resuscitation and subculture, as well as biochemical tests of various bacteria. This product is pale yellow dry powder made from refined raw materials with a well-proportioned formula and excellent solubility. After reconstitution, the liquid is clear and transparent without precipitates or impurities, featuring a mild system free of bacteriostatic ingredients. The medium is rich in nitrogen sources, organic matter, minerals and growth factors required for bacterial proliferation.
Basic Information:
Product Name | General Broth Medium | Catalog No. | R1097610 | Specification | 250g per bottle | Appearance | Pale yellow powder, odorless | Application | For enrichment culture of Staphylococcus aureus and other bacteria | Storage | Sealed, dry, dark place at room temperature | Shelf Life | 3 years |
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Formula:
Ingredient | Dosage (g) | Peptone | 20.0 | Beef Powder | 5.0 | Sodium Chloride | 5.0 | Final pH (25°C) | 7.5±0.1 |
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Preparation Method:
Weigh 30.0 g of the product and dissolve in 1 L of distilled water. Heat to boiling until fully dissolved, dispense into containers, autoclave at 121°C for 15 minutes, and set aside for use.
Quality Control:
Quality Control Strains and Strain Numbers
| Inoculum (cfu)
| Growth Performance
| Colony Character / Biochemical Reaction
| Staphylococcus aureus ATCC6538
| 10-100
| Good
| Turbidity
| Escherichia coli ATCC8739
| 10-100
| Good
| Turbidity
| Staphylococcus aureus ATCC25923
| 10-100
| Good
| Turbidity
| Salmonella Typhimurium ATCC14028
| 10-100
| Good
| Turbidity
|
Note: Incubate at 30–35 °C for 18–24 hours. |
Precautions:
1. Wear proper protective gear while weighing the medium to prevent physical discomfort.
2. Store medium in a dry and dark environment. Tighten the bottle cap immediately after use to prevent moisture absorption and caking.
3. Waste medium must be autoclaved at 121°C for 30 minutes before disposal.
4. This product is not intended for clinical testing.
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