GLS2 Mouse mAb - Primärer Antikörper, specific to GLS2

Cat. No.: Ab105528

GRADE & PURITY See COA

Application

IHC, WB

Host species

Maus

Reactive species

Maus, Mensch

Isotype

Mouse IgG1

Clone Number

H58S879

Conjugation

Unconjugated

Übersicht

Mouse anti Human GLS2 Antibody, Monoclonal (H58S879), could be used for WB, IHC and so on.

Specifications

Product Name

GLS2 Mouse mAb - Primärer Antikörper, specific to GLS2

Synonyme

breast cell glutaminase antibody | GA antibody | GLS antibody | Gls2 antibody | GLSL_HUMAN antibody | glutaminase 2 (liver mitochondrial) antibody | glutaminase 2 antibody | glutaminase GA antibody | glutaminase I antibody | Glutaminase liver isoform anti

Spezifikationen & Reinheit

See COA

Biochemische und physiologische Mechanismen

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH

Wirtsspezies

Maus

Spezifität

GLS2

Immunogen

Recombinant protein of human GLS2

Positive Kontrolle

WB: Human brain, Mouse liver lysates. IHC: Human kidney tissue.

Konjugation

Unconjugated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: GLS2 Mouse mAb (Ab105528)

GLS2 Mouse mAb (Ab105528) - Western Blot 

All lanes: GLS2 Mouse mAb (Ab105528) at 1/500 dilution Samples: Lysates at 20 µg per lane 

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution 

Predicted band size: 66 kDa 

Observed band size: 66 kDa 

Exposure time: 60.0 s

IHC: GLS2 Mouse mAb (Ab105528)

GLS2 Mouse mAb (Ab105528) - IHC Immunohistochemical analysis of GLS2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GLS2 Mouse mAb (Ab105528) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anwendung

• Anwendung: Dilution info
• IHC:

  1/50-1/200;

• WB:

  1/500-1/1000 ;

Zugehörige Ziele (menschlich)

GLS2 Tchem Glutaminase liver isoform, mitochondrial (0 Activities)

Discover Target: GLS2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GLS2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.