Glucosinolate Content Assay Kit (WST-1, Micro Method)

Cat. No.: G1527265

GRADE & PURITY BioReagent

Übersicht

Myrosinase catalyzes the hydrolysis of glucosinolates to produce glucose. The glucose is then sequentially catalyzed by hexokinase (HK) and glucose‑6‑phosphate dehydrogenase (G6PDH) to generate 6‑phosphogluconate, with concomitant reduction of NADP⁺ to NADPH. The NADPH, via electron transfer mediated by 1‑mPMS, reduces WST‑1 to formazan, a product that exhibits a characteristic absorption peak at 450 nm. The glucosinolate content can be quantitatively determined by monitoring the change in absorbance.

Specifications

Spezifikationen & Reinheit

BioReagent

Note

BioReagent

Detection instrument

Microplate reader

Detection wavelength

450nm

Lagerung und Versand

Storage

Store at 2-8°C

Verschickt in

Wet ice