Glycogen Phosphorylase b (GPb) Activity Assay Kit (UV Micro Method)

Cat. No.: G1521925

GRADE & PURITY BioReagent

Übersicht

Glycogen phosphorylase (GP, EC 2.4.1.1) is a key enzyme in glycogen catabolism. It cleaves α-1,4-glycosidic bonds one by one from the non-reducing ends of the glycogen molecule, removing glucosyl residues and releasing glucose-1-phosphate, until it reaches four glucose residues before the α-1,6-glycosidic branch points. GP exists in two forms: the active glycogen phosphorylase a (GPa) and the inactive glycogen phosphorylase b (GPb). GPb can be activated in the presence of a certain concentration of adenylate (5'-AMP). Determination Principle: GP catalyzes the reaction between glycogen and inorganic phosphate, producing a shortened glycogen molecule and glucose-1-phosphate. Phosphoglucomutase and glucose-6-phosphate dehydrogenase subsequently catalyze the reduction of NADP⁺ to NADPH. GP activity is determined by measuring the rate of increase in NADPH at 340 nm. GP (GPa and GPb) activity is measured in the presence of a certain concentration of adenylate (5'-AMP), while GPa activity is measured in the absence of adenylate (5'-AMP). GPb activity is obtained by subtracting GPa activity from total GP activity.

Specifications

Spezifikationen & Reinheit

BioReagent

Note

BioReagent

Anwendung

Enzyme activity assay

Detection instrument

Microplate reader,UV Spectrophotometer

Detection wavelength

340nm

Lagerung und Versand

Storage

Store at 2-8°C

Verschickt in

Wet ice

Stabilität und Lagerung

Store at 2-8℃ for 6 months.