GRP94 Antibody - Primärer Antikörper, specific to HSP90B1, Rabbit IgG

Cat. No.: Ab106543

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, See COA

Application

IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human GRP94 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.01-2µg/mL
IHC: 5-20µg/mL
Protein function
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.

Specifications

Product Name

GRP94 Antibody - Primärer Antikörper, specific to HSP90B1, Rabbit IgG

Synonyme

94 kDa glucose-regulated protein | ECGP | Endoplasmin | endothelial cell (HBMEC) glycoprotein | glucose regulated protein, 94 kDa | gp96 | GP96ECGP | Grp94 | GRP-94 | GRP94gp96 homolog | Heat shock protein 90 kDa beta member 1 | heat shock protein 90kDa b

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Wirtsspezies

Kaninchen

Spezifität

HSP90B1

Immunogen

Recombinant HSP90b1 expressed in E.coli (AA 22-502)

Positive Kontrolle

WB: HEK293, Hep G2, MCF7, Jurkat, HeLa, K562, NIH/3T3 and C6 cell lysates. IHC: Mouse cerebrum, testis, liver, kidney tissues.

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: GRP94 Antibody (Ab106543)

GRP94 Antibody (Ab106543) - Western Blot
All lanes: GRP94 Antibody (Ab106543) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 94 kDa
Observed band size: 100 kDa
Exposure time: 21.5 s

IHC: GRP94 Antibody (Ab106543)

GRP94 Antibody (Ab106543) - IHC
DAB staining on IHC
Sample: Mouse Cerebrum Tissue
Primary Ab: 20 µg/mL GRP94 Antibody (Ab106543)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GRP94 Antibody (Ab106543)

GRP94 Antibody (Ab106543) - IHC
DAB staining on IHC
Sample: Mouse Testis Tissue
Primary Ab: 20 µg/mL GRP94 Antibody (Ab106543)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GRP94 Antibody (Ab106543)

GRP94 Antibody (Ab106543) - IHC
DAB staining on IHC
Sample: Mouse Liver Tissue
Primary Ab: 20 µg/mL GRP94 Antibody (Ab106543)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GRP94 Antibody (Ab106543)

GRP94 Antibody (Ab106543) - IHC
DAB staining on IHC
Sample: Mouse Kidney Tissue
Primary Ab: 20 µg/mL GRP94 Antibody (Ab106543)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GRP94 Antibody (Ab106543)

GRP94 Antibody (Ab106543) - IHC
DAB staining on IHC
Sample: Mouse Cerebrum Tissue
Primary Ab: 20 µg/mL GRP94 Antibody (Ab106543)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GRP94 Antibody (Ab106543)

GRP94 Antibody (Ab106543) - IHC
DAB staining on IHC
Sample: Mouse Testis Tissue
Primary Ab: 20 µg/mL GRP94 Antibody (Ab106543)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GRP94 Antibody (Ab106543)

GRP94 Antibody (Ab106543) - IHC
DAB staining on IHC
Sample: Mouse Liver Tissue
Primary Ab: 20 µg/mL GRP94 Antibody (Ab106543)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Anwendung

• Anwendung: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.01-2 µg/mL ;

Zugehörige Ziele (menschlich)

HSP90B1 Tchem Endoplasmin (0 Activities)

Discover Target: HSP90B1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GRP94 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.