GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types.
Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting
Host species
Mouse
Specificity
Human HGF
Immunogen
Recombinant protein corresponding to Human HGF (D384N, D416N, L622S, H645R, V678I)
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Matched Pairs
Ab232189, HGF Mouse mAb, Capture Antibody
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Sandwich ELISA - HGF standard curve Sandwich ELISA detection of Recombinant Human HGF Protein (rp175908) using HGF Mouse mAb (Ab232189) as capture antibody at concentration of 2.0 µg/mL. Biotinylated HGF Mouse mAb (Ab169020) as detection antibody at concentration of 1.0 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.1 ng/mL - 2000.0 ng/mL
Indirect ELISA - HGF standard curve Immobilized Recombinant Human HGF Protein (rp175908) at 1.0 µg/mL overnight at 4℃. Coated protein probed with HGF Mouse mAb (Ab169020). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC₅₀: 30.12 ng/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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