HMGA2 Antibody - Primärer Antikörper, specific to HMGA2, Rabbit IgG

Cat. No.: Ab108293

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human HMGA2 Antibody, Polyclonal, could be used for WB, Flow and so on.
Application:
WB: 1/1000
Flow: 1/10-1/50
Protein Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
Post-translational
Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.

Specifications

Product Name

HMGA2 Antibody - Primärer Antikörper, specific to HMGA2, Rabbit IgG

Synonyme

High mobility group AT-hook protein 2 | HMGA 2 antibody | BABL antibody | High mobility group (nonhistone chromosomal) protein isoform I C antibody | High mobility group (nonhistone chromosomal) protein isoform IC antibody | High Mobility Group AT hook 2

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Wirtsspezies

Kaninchen

Spezifität

HMGA2

Immunogen

A KLH conjugated synthetic peptide from the C-terminal region of human HMGA2 (AA 64-92).

Positive Kontrolle

WB: Hep G2, NIH/3T3 and C6 cell lysates. Flow: Hep G2 cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified + Peptide affinity purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: HMGA2 Antibody (Ab108293)

HMGA2 Antibody (Ab108293) - Western Blot
All lanes: HMGA2 Antibody (Ab108293) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 16, 19 kDa
Exposure time: 120.0 s

Western Blot: HMGA2 Antibody (Ab108293)

HMGA2 Antibody (Ab108293) - Western Blot
All lanes: HMGA2 Antibody (Ab108293) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 16, 19 kDa
Exposure time: 120.0 s

Western Blot: HMGA2 Antibody (Ab108293)

HMGA2 Antibody (Ab108293) - Flow

HMGA2 Antibody (Ab108293) flow cytometric analysis of Hep G2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anwendung

• Anwendung: Dilution info
• WB:

  1/1000 ;

• Flow Cytometry:

  1/10-1/50;

Zugehörige Ziele (menschlich)

HMGA2 Tbio High mobility group protein HMGI-C (0 Activities)

Discover Target: HMGA2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MFAP5 Tbio Microfibrillar-associated protein 5 (0 Activities)

Discover Target: MFAP5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HMGA2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.