Human BMP-9 ELISA Kit

Cat. No.: H1509769

QUALITÄT & REINHEIT BioReagent

Übersicht

This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human BMP-9 concentration in samples. The Human BMP-9 capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human BMP-9 present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human BMP-9 is added. This antibody binds to the captured Human BMP-9, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human BMP-9 is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman BMP-9 is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman BMP-9, and the concentration of Human BMP-9 in unknown samples is interpolated from this curve based on their OD values.

Spezifikationen

Spezifikationen & Reinheit

BioReagent

Note

BioReagent

Anwendung

ELISA

Probentyp

Gewebehomogenate, Körperflüssigkeiten, Plasma, Serum, Zellkulturüberstände

Erfassungsbereich

15.62 pg/mL~1,000 pg/mL

Methode des Nachweises

Sandwich ELISA

Erkannte Art Objekt

Human

Spezifität

Detecting Human BMP-9 in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Lagerung und Versand

Storage

Store at 2-8°C

Verschickt in

Wet ice

Stabilität und Lagerung

Store at 2-8℃ for 6 months.

Häufig gestellte Fragen

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.