Factor VIIa, CAS No.65312-43-8

CAS: 65312-43-8 Cat. No.: H1442061

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), from Human plasma; Pre-lyophilization Protein Concentration: See COA

Accession #

P08709

Bioactivity

See COA

Overview

  Prepared from purified Human Factor VII using Human Factor XIIa. The Factor Xlla is removed using affinity chromatography. Purity is determined by SDS-PAGE. Human Factor VIIa reduces to 29,500 and 23,500 with the addition of 2-mercaptoethanol. Activity is determined via clotting assay. Factor Vlla, in the presence of calcium ions and Tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa.

  Extinction Coefficient (1%) = 13.9

  The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.

Specifications

Product Name

Factor VIIa, CAS No.65312-43-8

Synonyms

Human Factor VIIa Native Protein | Coagulation Factor VIIa, Human Plasma | coagulation factor VIIa | Coagulation factor VII | F7 | SPCA

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), from Human plasma; Pre-lyophilization Protein Concentration: See COA

Biochemical and Physiological Mechanisms

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue

Bioactivity

See COA

Accession #

P08709

Predicted molecular weight

50 kDa

CAS

65312-43-8

Enzyme Commission Number

EC 3.4.21.21

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Human plasma; Pre-lyophilization Protein Concentration: See COA

Reconstitution

We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term. Upon reconstitution, it is recommended to aliquot and freeze at -80℃.