Human IgG (Biotin) - Isotyp-Kontrollantikörper, IgG

Cat. No.: Ab180314

GRADE & PURITY ExactAb™, Validated, See COA

Application

Flow Cytometry

Host species

Mensch

Isotype

Human IgG

Conjugation

Biotin

Übersicht

Application:
Flow: ≤0.5 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

Human IgG (Biotin) - Isotyp-Kontrollantikörper, IgG

Synonyme

Biotin-conjugated Human Polyclonal antibody | Biotin Human IgG - Isotype Control | Human IgG

Spezifikationen & Reinheit

ExactAb™, Validated, See COA

Wirtsspezies

Mensch

Konjugation

Biotin

Note

ExactAb™, Validated

Isotyp

IgG

Verfahren zur Reinigung

Affinity chromatography

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

ELISA: Human IgG (Biotin) (Ab180314)

Human IgG (Biotin) (Ab180314) - ELISA
Human IgG (Biotin) (Ab180314) was tested by direct ELISA. The plate was coated with Goat Anti-Human IgG Antibody (Ab170447). A serial dilution of Human IgG (Biotin) (Ab180314) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Flow Cytometry: Human IgG (Biotin) (Ab180314)

Human IgG (Biotin) (Ab180314) - Flow Cytometry
Human peripheral blood lymphocytes are stained with Recombinant CD3 Antibody (APC) (Ab178034) and Recombinant CD73 Antibody (Biotin) (Ab176030) (Right), followed by Recombinant Streptavidin Protein (R-PE) (rp156150). Lymphocytes are stained with Recombinant CD3 Antibody (APC) (Ab178034) and Human IgG (Biotin) (Ab180314) (Left).

Anwendung

Anwendung

Dilution info

Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Human IgG (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.