BioReagent BioReagent für empfindliche chromatographische und analytische Workflows, die minimale Basislinieninterferenzen erfordern.
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This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human THBD concentration in samples. The Human THBD capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human THBD present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human THBD is added. This antibody binds to the captured Human THBD, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human THBD is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman THBD is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman THBD, and the concentration of Human THBD in unknown samples is interpolated from this curve based on their OD values.
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