Hyaluronidase used for Hyaluronidase from Streptomyces hyalurolyticus has been used: in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour

CAS: 37259-53-3 Cat. No.: H298749 EG-Nummer: 253-430-8

GRADE & PURITY EnzymoPure™, from Streptomyces hyalurolyticus; ≥300 U/vial

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used: in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue; as a component

Übersicht

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards. Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover. Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.

Specifications

Product Name

Hyaluronidase used for Hyaluronidase from Streptomyces hyalurolyticus has been used: in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour

Synonyme

Hyaluronate Lyase

Note

EnzymoPure™

Spezifikationen & Reinheit

EnzymoPure™, from Streptomyces hyalurolyticus; ≥300 U/vial

CAS

37259-53-3

Enzym Kommissionsnummer

4.2.2.1 (BRENDA, IUBMB)

Molekültyp

Enzyme

Lagerung und Versand

Anmeldung

Hyaluronidase from Streptomyces hyalurolyticus has been used: in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue; as a component

Konzentration

from Streptomyces hyalurolyticus; ≥300 U/vial

Storage

Store at -20°C

Verschickt in

Ice chest + Ice pads

Definition der Einheit

One unit will degrade 0.75μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 °C (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).