I-A/I-E Rat mAb (AF594) - Primärer Antikörper, specific to I-A/I-E

Cat. No.: Ab190266

GRADE & PURITY ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2b

Clone Number

M5/114

Conjugation

AF594

Übersicht

Rat anti Mouse I-A/I-E Antibody, Monoclonal (M5/114), could be used for Flow Cytometry.

Specifications

Product Name

I-A/I-E Rat mAb (AF594) - Primärer Antikörper, specific to I-A/I-E

Synonyme

IAb | Ia2 | Ia-2 | Abeta | H-2Ab | H2-Ab | Rmcs1 | I-Abeta | Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | E-alpha-f | H-2 class II histocompatibility antigen, E-K alpha chain | H-2 class II histocompatibility antigen, E-U alpha chain | H-2E | H2-Ea | H-2Ea | H2-Ea-ps

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides

Wirtsspezies

Rat

Spezifität

I-A/I-E

Immunogen

Activated C57BL/6 mouse spleen cells

Positive Kontrolle

Flow Cytometry: C57BL/6 mouse splenocytes.

Konjugation

AF594

Anregung (nm)

Yellow-Green Laser(594nm)

Emission (nm)

618nm

Ex/Em(nm)

Ex:590nm;Em:618nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: I-A/I-E Rat mAb (AF594) (Ab190266)

I-A/I-E Rat mAb (AF594) (Ab190266) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with I-A/I-E Rat mAb (AF594) (Ab190266) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF594) (Ab190241).

Flow Cytometry: I-A/I-E Rat mAb (AF594) (Ab190266)

I-A/I-E Rat mAb (AF594) (Ab190266) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with I-A/I-E Rat mAb (AF594) (Ab190266) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF594) (Ab190241). Black - Unlabelled control, cells without incubation with primary antibody.

Zugehörige Ziele (menschlich)

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Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (I-A/I-E Rat mAb (AF594)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.