I-A/I-E Rat mAb (APC) - Primärer Antikörper, specific to I-A/I-E, Rat IgG2b

Cat. No.: Ab190261

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2b

Clone Number

M5/114

Conjugation

Unconjugated

Übersicht

Ratten-Antimaus-I-A/I-E-Antikörper, monoklonal (M5/114), kann für Flow verwendet werden.

Specifications

Product Name

I-A/I-E Rat mAb (APC) - Primärer Antikörper, specific to I-A/I-E, Rat IgG2b

Synonyme

IAb | Ia2 | Ia-2 | Abeta | H-2Ab | H2-Ab | Rmcs1 | I-Abeta | Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | E-alpha-f | H-2 Klasse II Histokompatibilitätsantigen, E-K-Alpha-Kette | H-2 Klasse II Histokompatibilitätsantigen, E-U-Alpha-Kette | H-2E | H2-Ea | H-2Ea | H2-E

Spezifikationen & Reinheit

ExactAb™, validiert, 0,5 mg/mL

Biochemische und physiologische Mechanismen

Bindet Peptide, die von Antigenen stammen, die den endozytischen Weg der antigenpräsentierenden Zellen (APC) passieren, und präsentiert sie auf der Zelloberfläche zur Erkennung durch die CD4-T-Zellen. Der Peptidbindungsspalt kann Peptide mit 10-30 Resten

Wirtsspezies

Rat

Spezifität

I-A/I-E

Immunogen

Activated C57BL/6 mouse spleen cells

Positive Kontrolle

Flow: C57BL/6 mouse splenocytes.

Konjugation

Unconjugated

Note

ExactAb™, Validated

Isotyp

Rat IgG2b

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Bei 2-8°C lagern, vor Licht geschützt, einfrieren verboten

Verschickt in

Nasses Eis

Stabilität und Lagerung

Die Antikörperlösung sollte unverdünnt zwischen 2°C und 8°C gelagert und vor längerer Lichteinwirkung geschützt werden. Nicht einfrieren.

Bilder

Flow Cytometry: I-A/I-E Rat mAb (APC) (Ab190261)

I-A/I-E Rat mAb (APC) (Ab190261) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with I-A/I-E Rat mAb (APC) (Ab190261) at 0.012μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (APC) (Ab180313).

Flow Cytometry: I-A/I-E Rat mAb (APC) (Ab190261)

I-A/I-E Rat mAb (APC) (Ab190261) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with I-A/I-E Rat mAb (APC) (Ab190261) at 0.012μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  ≤0.1 μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume.;

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (I-A/I-E Rat mAb (APC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.