GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human ICAD Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-2 μg/mL
IHC: 5-20 μg/mL
IF/ICC: 2.5 μg/mL
Protein Function
Inhibitor of the caspase-activated DNase (DFF40).
Specifications
Product Name
ICAD Antibody - Primary antibody, specific to ICAD, Rabbit IgG
Carrier Free, ExactAb™, Validated, High Performance, See COA
Host species
Rabbit
Specificity
ICAD
Immunogen
Recombinant ICAD expressed in E.coli (AA 51-305).
Positive Control
WB: Jurkat, A431, Hep G2, K562 and MCF7 cell lysates. IHC: Human breast cancer and kidney tissues. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified + Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
ICAD Antibody (Ab109205) - Western Blot All lanes: ICAD Antibody (Ab109205) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa Observed band size: 41 kDa Exposure time: 10.0 s
ICAD Antibody (Ab109205) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL ICAD Antibody (Ab109205) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ICAD Antibody (Ab109205) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL ICAD Antibody (Ab109205) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ICAD Antibody (Ab109205) - IF/ICC
IF analysis of ICAD Antibody (Ab109205) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ICAD Antibody (Ab109205) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
2.5 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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