GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Übersicht
Rabbit anti Human IDH2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-2µg/mL
IHC: 5-20µg/mL
Protein Function
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Specifications
Product Name
IDH2 Antibody - Primärer Antikörper, specific to IDH2, Rabbit IgG
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Wirtsspezies
Kaninchen
Spezifität
IDH2
Immunogen
Recombinant IDH2 expressed in E.coli (AA 40-452).
Positive Kontrolle
WB: NIH/3T3, MCF7, Jurkat and Hep G2 cell lysates. IHC: Human stomach, kidney, glioma, liver cancer, prostate, liver tissues. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Antigen affinity purified + Protein A purified
Konzentration
See COA
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
IDH2 Antibody (Ab109347) - Western Blot All lanes: IDH2 Antibody (Ab109347) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 138.7 s
IDH2 Antibody (Ab109347) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 10 µg/mL IDH2 Antibody (Ab109347) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
IDH2 Antibody (Ab109347) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL IDH2 Antibody (Ab109347) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
IDH2 Antibody (Ab109347) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 10 µg/mL IDH2 Antibody (Ab109347) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
IDH2 Antibody (Ab109347) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 10 µg/mL IDH2 Antibody (Ab109347) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
IDH2 Antibody (Ab109347) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 10 µg/mL IDH2 Antibody (Ab109347) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
IDH2 Antibody (Ab109347) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 µg/mL IDH2 Antibody (Ab109347) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
IDH2 Antibody (Ab109347) - IF/ICC
IF analysis of IDH2 Antibody (Ab109347) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with IDH2 Antibody (Ab109347) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Anwendung
Anwendung
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IHC
5-20µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Bewertungen
Kundenbewertungen
Häufig gestellte Fragen
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IDH2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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