GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)1.0 mg/mL
Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208).
Also plays an important role in neutrophil activation (PubMe
Host species
Mouse
Specificity
Human IL8
Immunogen
Recombinant protein corresponding to Human IL8
Conjugation
Unconjugated
Grade
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Matched Pairs
Ab214004Z; IL-8 Mouse mAb（B05/6E2）；ELISA
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Sandwich ELISA - IL-8 standard curve
Sandwich ELISA detection of Recombinant Human IL-8 Protein (rp170400) using IL-8 Mouse mAb (Ab305305) as capture antibody at concentration of 1.0 µg/mL. Biotinylated IL-8 Mouse mAb (Ab214004) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 50.0 pg/mL - 4000.0 pg/mL
Indirect ELISA - IL-8 standard curve
Immobilized Recombinant Human IL-8 Protein (rp170400) at 1.0 µg/mL overnight at 4℃. Coated protein probed with IL-8 Mouse mAb (Ab305305). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC₅₀: 36.4 ng/mL
Application
Application
Dilution info
ELISA
1.0 µg/mL as a Capture antibody in ELISA
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
