GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)1.0 mg/mL
Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208).
Also plays an important role in neutrophil activation (PubMe
Wirtsspezies
Maus
Spezifität
Human IL8
Immunogen
Recombinant protein corresponding to Human IL8
Konjugation
Unconjugated
Note
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Lichterketten-Typ
kappa
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Protein A purified
Übereinstimmende Paare
Ab214004Z; IL-8 Mouse mAb（B05/6E2）；ELISA
Konzentration
1.0 mg/mL
Lagerung und Versand
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
Sandwich ELISA - IL-8 standard curve
Sandwich ELISA detection of Recombinant Human IL-8 Protein (rp170400) using IL-8 Mouse mAb (Ab305305) as capture antibody at concentration of 1.0 µg/mL. Biotinylated IL-8 Mouse mAb (Ab214004) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 50.0 pg/mL - 4000.0 pg/mL
Indirect ELISA - IL-8 standard curve
Immobilized Recombinant Human IL-8 Protein (rp170400) at 1.0 µg/mL overnight at 4℃. Coated protein probed with IL-8 Mouse mAb (Ab305305). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC₅₀: 36.4 ng/mL
Anwendung
Anwendung
Dilution info
ELISA
1.0 µg/mL as a Capture antibody in ELISA
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Zertifikate (CoA, COO, BSE/TSE und Analyse-Diagramm)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Lösungsrechner
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Bewertungen
Kundenbewertungen
Häufig gestellte Fragen
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does Animal Free mean?
Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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