Immunostaining Secondary Antibody Dilution Buffer - BioReagent, sterile-filtered, ready-to-use, Suitable for Immunohistochemistry, Suitable for Immunofluorescence

Cat. No.: I752114
AVAILABLE TO ORDER
GRADE & PURITY Sterile-filtered ? Sterile-filtered — passed through a 0.2µm filter to remove microorganisms. Use as a ready aseptic solution for culture and sensitive applications. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. Ready-to-use ? Ready-to-use — supplied pre-formulated at working concentration, no prep needed. Use to save time and reduce pipetting/dilution errors. Suitable for Immunohistochemistry(IHC) ? IHC grade — validated for immunohistochemistry on tissue sections. Use to detect and localize antigens in fixed tissue. Suitable for Immunofluorescence(IF) ? IF grade — validated for immunofluorescence with low background staining. Use to localize targets in cells/tissue by fluorescence microscopy.
Storage
Store at 2-8°C
Shipped In
Wet ice
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Size
Status
Price
Qty
100ml
I752114-100ml
2
$29.90
Enter a quantity for the sizes you want to add.
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Why this grade

BioReagent, sterile-filtered, ready-to-use, Suitable for Immunohistochemistry, Suitable for Immunofluorescence BioReagent,Ready-to-use,Sterile-filtered,Suitable for Immunofluorescence(IF),Suitable for Immunohistochemistry(IHC) for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

This product is an optimized antibody diluent for immunostaining on the basis of traditional second antibody diluent, which can be used for the dilution of second antibody in immunostaining experiments such as immunohistochemistry and immunocytochemistry.
Operation process:
1. Reagent preparation refers to the instructions for the use of the second antibody used, as well as the quality of the primary antibody and the content of the target protein in the sample, diluting the second antibody in accordance with the appropriate proportion, such as 1:1000, 1:500 and other proportions.
2. Antibody incubation The second antibody can be incubated at the appropriate temperature after dilution of this product and directly used for immunostaining.
3, color reaction according to the needs of the experiment, choose the appropriate color reagent for color development.
Note: 
1. This product has been filtered treatment, once started, it is recommended to use within one month, if found precipitate or bacterial contamination please discard. 
2. The second antibody diluted by this product can be reused 3 to 5 times within 1 to 2 weeks under appropriate storage conditions. Tips For your own safety, before using the reagent, please take good protection, such as wearing lab clothes, gloves, etc.

Specifications

Specifications & Purity
BioReagent, sterile-filtered, ready-to-use, Suitable for Immunohistochemistry, Suitable for Immunofluorescence
Stability And Storage
Store at 2-8℃ for 12 months. Store at -20℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Storage
Store at 2-8°C
Shipped In
Wet ice
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent, Ready-to-use, Sterile-filtered, Suitable for Immunofluorescence(IF), Suitable for Immunohistochemistry(IHC)

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot NumberCertificate TypeDateItem
ZJ25F0420224Certificate of AnalysisFeb 24, 2026 I752114
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