Ixekizumab ELISA Kit

Cat. No.: EJ1515736

QUALITÄT & REINHEIT BioReagent

Übersicht

This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human IL-17A antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Ixekizumab in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.

Spezifikationen

Spezifikationen & Reinheit

BioReagent

Note

BioReagent

Anwendung

ELISA

Probentyp

Gewebehomogenate, Plasma, Serum, Zellkulturüberstände, Zelllysat

Erfassungsbereich

0.78ng/mL~50ng/mL

Erkannte Art Objekt

Human

Spezifität

Detecting Human Ixekizumab in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

Lagerung und Versand

Storage

Store at 2-8°C

Verschickt in

Wet ice

Stabilität und Lagerung

Store at 2-8℃ for 6 months.