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In 0 peer-reviewten Publikationen zu Chromatographie, organischer Synthese und Kreuzkupplungsreaktionen zitiert.
Agarose bound Jacalin is prepared using our affinity-purified lectins. Jacalin is a lectin composed of four subunits of approximately 16 kDa each. This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or desialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains. Features: • Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2×10^7 daltons • Bead diameter ranges in size from 45-165 microns • Matrix is stable in solutions at pH 3-11 as well as many organic solvents • Immobilized lectins are prepared using affinity purified lectins • Product supplied as a 1:1 suspension in buffer Technical Information Agarose bound* Jacalin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix. This coupling method provides several advantages over the traditional cyanogen bromide procedure: Maximum carbohydrate binding activity of the coupled lectins is retained Linkage is stable over a range of pH values Conjugated proteins are not leached off the beads by Tris or other routinely used buffers No residual charges are present after conjugation. This minimizes non-specific binding to the matrix Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins. Inhibiting/Eluting Sugar: 800 mM galactose or 100 mM melibiose or Glycoprotein Eluting Solution (ES-2100) *4 mg lectin/ml gel
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