JMJD6 Antibody - Primärer Antikörper, specific to JMJD6, Rabbit IgG

Cat. No.: Ab111495

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, See COA

Application

WB

Host species

Kaninchen

Reactive species

Mensch

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human JMJD6 Antibody, Polyclonal, could be used for WB and so on.
Application
WB: 0.01-2µg/mL
Function
Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.

Specifications

Product Name

JMJD6 Antibody - Primärer Antikörper, specific to JMJD6, Rabbit IgG

Synonyme

EC 1.14.11.- | Histone arginine demethylase JMJD6 | JmjC domain-containing protein 6 | Jumonji domain-containing protein 6 | Lysyl-hydroxylase JMJD6 | Peptide-lysine 5-dioxygenase JMJD6 | Phosphatidylserine receptor | Protein PTDSR | Apoptotic cell cleara

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Azide Free, Validated, See COA

Wirtsspezies

Kaninchen

Spezifität

JMJD6

Immunogen

Recombinant PSR expressed in E.coli (AA 1-334).

Positive Kontrolle

WB: K562, Hep G2 and HEK293 cell lysates.

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Antigen affinity purified + Protein A purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: JMJD6 Antibody (Ab111495)

JMJD6 Antibody (Ab111495) - Western Blot
All lanes: JMJD6 Antibody (Ab111495) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 49 kDa
Exposure time: 32.2 s

Anwendung

Anwendung

Dilution info

WB

0.01-2 µg/mL

Zugehörige Ziele (menschlich)

JMJD6 Tchem Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 (0 Activities)

Discover Target: JMJD6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (JMJD6 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.