Dextrose Phosphate Broth (Powder)

Directions per Liter:

Dissolve 32 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Casein Digest Peptone	10
Yeast Extract	2
Sodium Chloride	5
Sodium Bisulfite	0.1
Peptic Digest of Animal Tissue	10
Dextrose	1
Disodum Phosphate	3
Sodium Citrate	1
	Total: 32g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.