Modified RIPA Buffer

Recipe

• 10 mM Tris, pH 7.4

• 100 mM NaCl

• 1 mM EDTA

• 1 mM EGTA

• 1 mM NaF

• 20 mM Na4P2O7

• 2 mM Na3VO4

• 1% Triton X-100

• 10% glycerol

• 0.1% SDS

• 0.5% deoxycholate

• plus protease inhibitors: Aprotinin, leupeptin, pepstatin: 1ug/ml each

• Add 1mM PMSF immediately before use.