Recombinant Human Annexin A10 Protein: FAQs & Key Facts

Recombinant Human Annexin A10 Protein is a research-grade recombinant protein representing a member of the annexin superfamily implicated in calcium-dependent membrane binding, intracellular signaling, and potential roles in gastrointestinal cancer biology.

Product Name

Recombinant Human Annexin A10 Protein

SKU

rp365052

Protein Family

Annexin superfamily (ANXA10)

Species Origin

Human (Homo sapiens)

Application

Research use only; suitable for studies in cell biology, cancer research, and proteomics

Storage Recommendation

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles (general guidance — verify with datasheet)

What Is Recombinant Human Annexin A10 Protein?

Annexin A10 (ANXA10) is a member of the annexin superfamily, a group of structurally related proteins characterized by their ability to bind phospholipid membranes in a calcium-dependent manner. The annexin superfamily plays diverse roles in cellular processes including membrane organization, vesicle trafficking, signal transduction, and regulation of cell growth and apoptosis.

Recombinant Human Annexin A10 Protein (SKU: rp365052) is produced using recombinant DNA technology to yield a highly pure protein suitable for in vitro research applications. ANXA10 has attracted scientific interest due to its reported expression patterns in gastrointestinal tissues and its potential relevance as a biomarker in certain cancer types, including gastric and colorectal cancers. This recombinant protein enables researchers to study ANXA10 function, protein–protein interactions, and its role in disease models without relying on primary tissue extraction.

Biological Function and Research Significance of ANXA10

Like other annexin family members, Annexin A10 contains the characteristic annexin repeat domains that mediate calcium-dependent phospholipid binding. These structural features suggest roles in membrane-associated processes and intracellular calcium signaling. While ANXA10 is less extensively characterized than some other annexin family members (such as ANXA1 or ANXA5), emerging evidence suggests it may participate in the regulation of cell proliferation and differentiation.

From a translational perspective, ANXA10 expression has been studied in the context of gastrointestinal cancers. Some research suggests that altered ANXA10 expression may correlate with tumor development or progression, positioning it as a candidate biomarker and potential therapeutic target. Recombinant ANXA10 protein is a valuable tool for researchers investigating these disease associations, enabling functional assays, antibody validation, and mechanistic studies.

Handling, Reconstitution, and Storage Guidelines

Proper handling of recombinant proteins is critical to maintaining biological activity and experimental reproducibility. The following general guidelines apply to Recombinant Human Annexin A10 Protein; always consult the product-specific datasheet (absea #PP-1585) for definitive instructions.

• Reconstitution: Lyophilized protein should typically be reconstituted in sterile deionized water or an appropriate buffer (e.g., PBS) to the recommended concentration. Allow the vial to equilibrate to room temperature before opening. Gently mix without vortexing to avoid denaturation.
• Storage: For long-term storage, aliquot the reconstituted protein and store at -80°C. For short-term use (up to 1 month), storage at -20°C is generally acceptable. Avoid repeated freeze-thaw cycles, which can reduce protein activity.
• Handling: Work on ice when possible. Use low-protein-binding tubes and pipette tips to minimize adsorption losses. Add a carrier protein such as BSA if the protein will be diluted to very low concentrations.

Note: Specific buffer composition, concentration, and storage conditions should be verified against the official product datasheet before use.

Recommended Research Applications

Recombinant Human Annexin A10 Protein is suitable for a range of in vitro research applications. Typical uses include:

• ELISA and immunoassay development: Use as a standard or coating antigen to develop or validate ANXA10-specific assays.
• Antibody validation: Serve as a positive control antigen to confirm antibody specificity in Western blot, ELISA, or immunoprecipitation experiments.
• Protein–protein interaction studies: Employ in pull-down assays, surface plasmon resonance (SPR), or co-immunoprecipitation to map ANXA10 binding partners.
• Functional and cell-based assays: Investigate the effect of exogenous ANXA10 on cellular processes such as proliferation, apoptosis, or membrane dynamics.
• Biomarker research: Use as a reference material in studies characterizing ANXA10 as a potential diagnostic or prognostic biomarker in gastrointestinal cancers.

This product is intended for research use only and is not approved for diagnostic or therapeutic use in humans or animals.

Quality, Purity, and Regulatory Information

Recombinant Human Annexin A10 Protein (rp365052) is manufactured to research-grade quality standards. General quality attributes for recombinant proteins of this type include:

• Purity: Recombinant proteins from reputable suppliers are typically produced to ≥90% purity as assessed by SDS-PAGE. Specific purity data for rp365052 should be confirmed on the product datasheet.
• Endotoxin levels: Low endotoxin formulations are important for cell-based assays. Consult the datasheet for the endotoxin specification for this product.
• Biological activity: Where applicable, activity is confirmed by functional assay. Check the datasheet for activity testing information specific to rp365052.
• Regulatory status: This product is for research use only (RUO). It is not a medicinal product and has not been evaluated by regulatory authorities such as the FDA or EMA for clinical use.

For complete specifications, certificates of analysis (CoA), and safety data sheets (SDS), please contact Aladdin Scientific or refer to the product page for SKU rp365052.

Frequently asked questions

What is Recombinant Human Annexin A10 Protein used for in research?

Recombinant Human Annexin A10 Protein (rp365052) is used in a variety of in vitro research applications, including ELISA development, antibody validation (e.g., as a positive control in Western blot or immunoprecipitation), protein–protein interaction studies, and functional cell-based assays. It is also used in biomarker research investigating ANXA10's role in gastrointestinal cancers. This product is for research use only and is not intended for diagnostic or therapeutic purposes.

How should I store and reconstitute Recombinant Human Annexin A10 Protein?

As a general guideline, lyophilized recombinant proteins should be reconstituted in sterile deionized water or an appropriate buffer (such as PBS) and gently mixed without vortexing. For long-term storage, aliquot the reconstituted protein and keep at -80°C; for short-term use, -20°C is typically acceptable. Repeated freeze-thaw cycles should be avoided to preserve protein activity. Always refer to the official product datasheet (absea #PP-1585) for product-specific reconstitution volumes, buffer recommendations, and storage conditions.

What is the purity of Recombinant Human Annexin A10 Protein (rp365052)?

Research-grade recombinant proteins are generally produced to high purity levels, typically ≥90% as assessed by SDS-PAGE, though the exact purity specification for rp365052 should be confirmed by consulting the product datasheet or certificate of analysis (CoA) available from Aladdin Scientific. If you require a CoA or purity data for a specific lot, please contact our technical support team.

What biological function does Annexin A10 (ANXA10) have?

Annexin A10 is a member of the annexin superfamily and shares the family's hallmark calcium-dependent phospholipid-binding activity mediated by conserved annexin repeat domains. It is thought to participate in membrane-associated signaling and intracellular calcium regulation. ANXA10 has been studied for its potential roles in gastrointestinal tissue biology and as a candidate biomarker in certain cancers, though its precise molecular functions continue to be an active area of research.

Is Recombinant Human Annexin A10 Protein suitable for use in cell-based assays?

Recombinant Human Annexin A10 Protein can be used in cell-based assays to investigate the effects of exogenous ANXA10 on cellular processes such as proliferation, apoptosis, or membrane dynamics. For cell-based applications, it is important to use a formulation with a low endotoxin level to avoid confounding effects on cell viability and signaling. Please consult the product datasheet for endotoxin specifications and recommended working concentrations for rp365052.

How do I order Recombinant Human Annexin A10 Protein from Aladdin Scientific?

Recombinant Human Annexin A10 Protein is available from Aladdin Scientific under SKU rp365052. You can place an order directly through the Aladdin Scientific website by searching for the SKU or product name, or by contacting our sales team. For bulk quantities, custom formulations, or pricing inquiries, please reach out to our customer service or your regional sales representative.

References

1. Moss SE, Morgan RO. The annexins. Genome Biology. 2004;5(4):219. doi:10.1186/gb-2004-5-4-219
2. Gerke V, Creutz CE, Moss SE. Annexins: linking Ca2+ signalling to membrane dynamics. Nature Reviews Molecular Cell Biology. 2005;6(6):449-461. doi:10.1038/nrm1661
3. absea Product Reference #PP-1585. Recombinant Human Annexin A10 Protein. Aladdin Scientific (rp365052).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.