Recombinant Human GAPDHS Protein: FAQs & Key Facts

Recombinant Human GAPDHS Protein is a research-grade recombinant form of the testis-specific glycolytic enzyme GAPDHS, produced for use in biochemical assays, functional studies, and related life science research applications.

Product Name

Recombinant Human GAPDHS Protein

SKU

rp367917

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant protein

Gene Name

GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic)

Supplier

Aladdin Scientific

What Is Recombinant Human GAPDHS Protein?

GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic) is a testis-specific isoform of the classical glycolytic enzyme GAPDH. Unlike the ubiquitously expressed GAPDH, GAPDHS expression is restricted primarily to spermatogenic cells, where it plays an essential role in sperm energy metabolism and motility. Recombinant Human GAPDHS Protein (SKU: rp367917) is a recombinant form of this enzyme produced for research applications, enabling scientists to study its biochemical properties, interactions, and potential as a target in male fertility research.

Applications and Research Uses

Recombinant Human GAPDHS Protein is suitable for a range of in vitro research applications. Typical uses include:

• Enzyme activity assays: Measuring GAPDHS-specific glycolytic activity in controlled biochemical systems.
• Protein interaction studies: Investigating binding partners relevant to sperm metabolism and motility pathways.
• Antibody validation: Serving as a positive control antigen for GAPDHS-specific antibodies in Western blot, ELISA, and related immunoassays.
• Drug discovery: Screening inhibitors or modulators targeting GAPDHS as a potential contraceptive or fertility-related therapeutic target.
• Structural and functional studies: Supporting structural biology investigations of spermatogenic GAPDH.

Researchers should verify suitability for specific assay formats prior to use.

Storage, Handling, and Stability

Proper storage and handling are critical for maintaining the activity and integrity of recombinant proteins. General best-practice guidelines for Recombinant Human GAPDHS Protein include:

• Storage temperature: Store at -20°C or -80°C as recommended on the product datasheet. Avoid repeated freeze-thaw cycles, which can degrade protein integrity and reduce activity.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize freeze-thaw events.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer specified in the product datasheet. Allow the vial to equilibrate at room temperature briefly before reconstitution.
• Short-term storage: For use within a few days, the reconstituted protein may be stored at 4°C; consult the datasheet for confirmed stability windows.

Always refer to the specific product datasheet (absea#PP-10722) provided with the product for confirmed storage conditions, buffer composition, and shelf-life information.

Ordering and Product Information

Recombinant Human GAPDHS Protein is available from Aladdin Scientific under SKU rp367917. To place an order or obtain a quotation, contact Aladdin Scientific through the official website or reach out to your regional sales representative. When ordering, please confirm:

• The required quantity and pack size.
• Any specific quality documentation needs (e.g., Certificate of Analysis, SDS).
• Shipping and cold-chain requirements, as recombinant proteins require temperature-controlled logistics.

For bulk or custom requests, contact the Aladdin Scientific sales team directly. Pricing, availability, and promotional offers may vary; please consult the current product listing for up-to-date information.

Frequently asked questions

What is GAPDHS and how does it differ from GAPDH?

GAPDHS is a spermatogenic, testis-specific isoform of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) that is expressed predominantly in sperm cells, where it supports energy production via glycolysis for sperm motility. In contrast, the canonical GAPDH is ubiquitously expressed across most cell types and tissues. GAPDHS contains an additional proline-rich N-terminal domain absent in somatic GAPDH, which anchors it to the fibrous sheath of the sperm flagellum.

What research applications is Recombinant Human GAPDHS Protein (rp367917) suitable for?

Recombinant Human GAPDHS Protein is suitable for enzyme activity assays, protein-protein interaction studies, antibody validation (e.g., as an antigen in Western blot or ELISA), drug discovery screening (particularly for male contraceptive targets), and structural biology research. Researchers are advised to verify suitability for their specific assay format by consulting the product datasheet or contacting Aladdin Scientific technical support.

How should Recombinant Human GAPDHS Protein be stored to maintain stability?

As a general best practice for recombinant proteins, this product should be stored at -20°C or -80°C, and repeated freeze-thaw cycles should be avoided by preparing single-use aliquots upon first thaw. Please refer to the specific product datasheet (absea#PP-10722) supplied with your order for confirmed storage temperatures, buffer formulation, and recommended shelf-life details, as these specifications take precedence over general guidelines.

Can Recombinant Human GAPDHS Protein be used as a positive control in immunoassays?

Yes, recombinant proteins such as GAPDHS are commonly used as positive control antigens in immunoassays including Western blot and ELISA, to validate antibody specificity and assay performance. Confirm that the protein preparation is compatible with your specific assay conditions by reviewing the datasheet or contacting Aladdin Scientific technical support.

How do I order Recombinant Human GAPDHS Protein from Aladdin Scientific?

You can order Recombinant Human GAPDHS Protein (SKU: rp367917) directly through the Aladdin Scientific website or by contacting your regional Aladdin Scientific sales representative. For bulk quantities or custom requests, please reach out to the sales team directly. Ensure you confirm pack size, required documentation, and cold-chain shipping needs at the time of ordering.

References

1. Bunch DO, et al. Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution during mouse spermatogenesis. Biology of Reproduction. 1998.
2. Miki K, et al. Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility. Proceedings of the National Academy of Sciences. 2004.
3. Aladdin Scientific. Recombinant Human GAPDHS Protein, SKU rp367917, Product Datasheet absea#PP-10722.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.