Lactobacillus Selective Broth (LBS Broth) - BioReagent

Cat. No.: L1528484

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Übersicht

For selective cultivation of lactic acid bacteria; 1.32 mL of glacial acetic acid must be added per liter of medium

Specifications

Spezifikationen & Reinheit

BioReagent

Storage

Room temperature

Verschickt in

Normal

Note

BioReagent

Product Line

Biologische Produkte

Application Protocols

General, literature-based guidance. Adjust per your SOP and validate with your strains and equipment. This product is for research use only.

1. Preparation of LBS Broth

• Use purified/deionized water. Gradually add dehydrated powder with stirring; heat gently until fully dissolved.
• Distribute into suitable vessels with appropriate headspace for gas exchange or anaerobic systems.
• Sterilize by autoclaving using a validated cycle. Allow to cool to handling temperature. Verify pH if your method requires it.

2. Inoculation and Incubation

• Inoculate aseptically with sample or starter culture. For enrichment from complex matrices, consider serial dilution or pre-enrichment strategies.
• Incubate at 30–37 °C (strain-dependent) for 24–72 h. For microaerophilic/anaerobic strains, use gas-generating sachets, jars, or an anaerobic workstation; pre-reduce medium if required.

3. Monitoring Growth

• Observe turbidity, measure OD600, track pH decrease, and/or plate onto selective agar for enumeration/isolation.
• Include controls: uninoculated sterility control; positive control with a known Lactobacillus strain; negative control with a non-target organism to assess selectivity.

4. Downstream Handling

• For isolation, streak from enriched broth onto appropriate selective agar.
• For metabolite analysis, centrifuge to remove cells; analyze supernatant (e.g., organic acids) by your validated method.

5. Disposal

• Autoclave spent media and cultures before disposal; follow institutional biosafety procedures.

Biological Roles

Context for Lactobacillus (general, literature):

• Lactobacillus spp. (now split into multiple genera taxonomically) are Gram-positive, lactic acid–producing bacteria central to many fermentations and microbiome niches. They metabolize carbohydrates primarily via homolactic or heterolactic fermentation, acidifying their environment.
• Selective broths like LBS provide nutrients and environmental conditions (notably acidic pH and selective inhibitors) that favor these organisms while suppressing competitors.

Research uses enabled by LBS-type broths:

• Enrichment from complex matrices prior to isolation on selective agar plates.
• Studying carbohydrate utilization, acid tolerance, and stress responses under controlled nutrient and pH conditions.
• Preparing biomass for downstream omics (genomics, transcriptomics, metabolomics) and measuring lactic acid production or other metabolites via HPLC/enzymatic assays.
• Evaluating tolerance to inhibitors or antibiotics in food and beverage process control contexts.

Notes:

• Growth kinetics in selective broth depend on species/strain, inoculum, pH, oxygen availability, and medium composition (literature). Establish in-house growth curves for your strains of interest.
• This product is for research and laboratory quality control only. It is not intended for clinical diagnostics or therapeutic use.

Buffer Applications

This product is a complete culture medium rather than a buffering reagent. It is not typically used to make defined laboratory buffers.

• If a defined buffer is required for your assay, prepare it separately and, if necessary, add to cultures post-growth. Validate any buffer additions for impact on selectivity and growth (literature).
• The formulation generally provides inherent buffering capacity suitable for Lactobacillus cultivation; altering it can change selectivity.

Green Alternatives

For culture media, “green alternatives” focus on process efficiency and waste minimization rather than solvent substitution.

Options and trade-offs (general):

• Ready-to-use sterile broths: Reduce energy/water use from in-house sterilization and minimize preparation variability. Trade-off: Increased packaging volume and shipping weight.
• Concentrate formats or bulk packaging: Reduce packaging waste per liter of prepared medium. Trade-off: Requires careful handling to avoid moisture uptake.
• Reusable glassware with validated cleaning/sterilization cycles: Lowers single-use plastics. Trade-off: Higher labor and energy for washing/autoclaving.
• Process optimization: Prepare only the volume needed; scale-down using mini-bioreactors or deep-well plates to reduce waste.
• Energy efficiency: If validated, use cycle-optimized autoclaving and avoid unnecessary extended sterilization times to lower energy use while maintaining sterility.

Chemical hazard profile:

• Dehydrated media typically have low intrinsic chemical hazard compared to organic solvents or strong reagents; primary risks stem from biohazard potential after inoculation. Employ appropriate biosafety and waste autoclaving to mitigate environmental release.

Pharmaceutical Uses

No therapeutic or clinical diagnostic uses are claimed. In pharmaceutical and biotech settings, LBS-type selective broths may be used in laboratory quality control workflows (general):

• Microbiological testing of raw materials, in-process samples, or finished products containing or susceptible to lactic acid bacteria, including probiotic formulations, under appropriate GMP/GLP procedures.
• Environmental monitoring and investigation of bioburden sources where lactic acid bacteria are relevant to process control.
• Strain banking and propagation for research into excipient interactions or stability of lactic cultures used in non-clinical studies.

Regulatory considerations:

• This BioReagent-grade product is for research/QC laboratory use only. If compendial conformance (e.g., pharmacopeial media) is required, verify suitability or use media specified by the applicable monograph or method.
• Maintain documentation of lot traceability, CoA, and any in-house growth promotion testing when used in regulated workflows.

Physical Properties

Item-specific physical constants are not defined for complex mixtures. Where applicable, consult the CoA/SDS.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Freely soluble in purified water (general for dehydrated media; literature).
• pH of prepared broth: Typically mildly acidic to favor Lactobacillus; exact pH for this item Not specified; refer to CoA/Spec Sheet. Typical literature target pH for LBS-type media after sterilization is around the mid-5 range.
• Density, refractive index, logP, BP/MP: Not applicable or not defined for aqueous prepared media; Not specified for this item; refer to CoA/Spec Sheet.

Notes for use (general, literature):

• Dissolve the dehydrated powder completely in water with gentle heating and stirring to obtain a clear to slightly opalescent solution before sterilization.
• Final pH can drift slightly after autoclaving; verify and document pH at use temperature if process-critical.
• Osmolality and buffering capacity depend on formulation; if downstream assays are pH-sensitive, verify with in-house QC.

All numerical specifications for this catalog item should be verified from the item’s CoA/Spec Sheet.

Quality and Grades

• Grade: BioReagent (as listed). This denotes suitability for research and laboratory applications, emphasizing consistent performance and impurity control appropriate for biological workflows. It is not produced to clinical diagnostic or pharmaceutical compendial standards unless otherwise stated.

What BioReagent means in practice:

• Suitable for routine microbiology, enrichment, and selectivity studies in research/QC contexts.
• Batch-to-batch consistency is emphasized so growth and selectivity characteristics remain reproducible under defined conditions.
• UV background, heavy metals, and residual solvents are typically not primary specification points for complex media; refer to the CoA/Spec Sheet for any lot-specific metrics. If a specific parameter is not provided there, it is not a controlled specification for this item.

Stabilizers and additives:

• No item-specific stabilizers are stated. Not specified for this item; refer to CoA/Spec Sheet.

Documentation and QC:

• For method validation or regulated environments, retain the lot CoA, SDS, and any vendor performance data (e.g., growth promotion testing, where available). If your SOP requires defined selectivity or growth indices, perform in-house verification with representative strains.

Shipping/storage:

• Shipped: Normal. Storage: Room temperature (per product data). Protect from moisture; tightly reseal after opening to maintain performance.

Reaction and Applications

This is a microbiological growth medium rather than a chemical reactant. Its applications center on selective cultivation and enrichment.

Key applications (general, literature):

• Selective enrichment and enumeration of Lactobacillus spp. from complex samples (e.g., fermented foods, probiotics, environmental swabs, process streams).
• Quality control in fermentation and food/beverage production: monitoring lactic acid bacteria presence/levels to assess starter performance or contamination control.
• Strain maintenance, propagation prior to downstream analyses (e.g., carbohydrate utilization tests, genomics, metabolomics).

Selectivity principles (general):

• Acidic pH and selective agents inhibit many Gram-negative bacteria and non-target flora while permitting lactic acid bacteria to grow.
• Rich carbon and nitrogen sources support fastidious Lactobacillus metabolic requirements; surfactants and acetate sources may enhance growth (literature for LBS-type media).

Practical tips:

• Pre-warm medium to incubation temperature to reduce lag phase.
• For obligately microaerophilic/anaerobic strains, use an anaerobic jar or workstation; pre-reduce medium if required.
• Media performance is sensitive to pH; verify final pH post-autoclave and adjust only if your method validation allows.
• To assess selectivity, include controls: a known Lactobacillus strain (positive control) and a non-target organism (negative control).

Note: All uses are for research or laboratory quality control only, not for clinical diagnostics or therapeutic applications.

Reaction Conditions

Not applicable to chemical reactions. However, culture conditions (general, literature) relevant to this medium are summarized for context:

• Atmosphere: Many Lactobacillus strains grow best under microaerophilic or anaerobic conditions. Use anaerobic jars, gas packs, or workstations as appropriate.
• Temperature: Common incubation ranges are 30–37 °C depending on species/strain. Validate optimal temperature for your isolates.
• Time: Enrichment typically proceeds for 24–72 hours, monitoring turbidity, pH drop, or CFU where required.
• pH: Selective media of this class are usually mildly acidic. Excessive neutralization may decrease selectivity.

All values above are general literature guidance and must be validated for your specific strains and SOPs.

Safety and Handling

Always consult the SDS for authoritative safety guidance. The following are general considerations for dehydrated microbiological media and cultures grown therein.

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• Likely hazards (general): Nuisance dust; may cause mechanical irritation to eyes and respiratory tract. Prepared media and used cultures can present biological hazards due to microbial growth; handle per biosafety procedures.
• PPE: Laboratory coat, safety glasses/goggles, and appropriate gloves. Use a dust mask or respirator if airborne dust may be generated. Employ biological safety cabinets when handling live cultures as dictated by organism risk group and institutional policy.
• Handling: Avoid creating dust; weigh in a ventilated enclosure when possible. Do not ingest. Prevent contamination of the container to maintain selectivity and performance.
• Incompatibilities: Strong oxidizers and reactive chemicals should be kept away from organic nutrient mixtures (general). Avoid prolonged exposure to humidity, which causes caking and performance loss.
• First aid (general): If inhaled, move to fresh air. If in eyes, rinse cautiously with water for several minutes. If on skin, wash with soap and water. If ingested, rinse mouth; seek medical advice. Always follow SDS.
• Sterilization/disposal: Autoclave spent media and cultures prior to disposal, following local biosafety and waste regulations.

Solvent Selection

This product is not a solvent; it is a dehydrated culture medium intended to be dissolved in water to prepare a selective broth.

Practical guidance (general, literature):

• Use purified/deionized water (microbiological grade). Elevated ionic contaminants or residual disinfectants (e.g., chlorine) can inhibit growth of Lactobacillus.
• Avoid organic cosolvents; they can disrupt selectivity and inhibit target organisms.
• Degassing is generally unnecessary; however, for microaerophilic or anaerobic cultivation, pre-reduce the medium and control headspace gases as appropriate.

Comparison (context):

• Water (preferred): Compatible with sterilization, preserves selectivity profile (literature).
• Buffers (e.g., phosphate) as primary solvent are typically unnecessary; buffering capacity is provided by the formulation (literature). If additional buffering is required for experimental design, validate impact on selectivity empirically.

Storage and Reconstitution

Item-specific storage and shipping:

• Storage Conditions: Room temperature (per product data). Protect from moisture and light. Keep container tightly closed; use desiccant where practical.
• Shipped In: Normal (per product data).

Reconstitution and preparation (general, literature):

• Use purified/deionized water. Add the dehydrated medium gradually with stirring; apply gentle heat to aid dissolution.
• Adjust volume accurately after dissolution. Do not adjust pH unless your validated method specifies; selectivity can be altered by pH changes.
• Dispense into suitable containers. Sterilize by autoclaving using a validated cycle. Allow to cool before use.
• For anaerobic applications, pre-reduce the sterilized medium by holding it under anaerobic conditions before inoculation.

Shelf life and stability:

• Unopened containers stored dry at room temperature are generally stable until the labeled expiration date. After opening, promptly reseal and minimize exposure to ambient humidity to prevent caking and performance drift. Lot-specific expiry and retest information: Not specified for this item; refer to CoA/Spec Sheet.

Freeze–thaw guidance:

• Not applicable. Store as a dry powder. Prepared liquid media are typically stored refrigerated for short periods if sterility can be assured; define limits in your SOP.

Always verify any critical parameters (e.g., pH, sterility) prior to use in regulated workflows.

Structure and Identity

This product is a complex, dehydrated culture medium formulated for the selective enrichment and cultivation of Lactobacillus spp. It is a mixture, not a single defined molecule.

• SKU: L1528484
• Product Name: Lactobacillus Selective Broth (LBS Broth)
• Item type: Dehydrated microbiological growth medium (mixture)
• Chemical identifiers (CAS, InChIKey, SMILES, CID): Not applicable to mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula and molecular weight: Not applicable to mixtures; Not specified for this item; refer to CoA/Spec Sheet.

Structural/functional features (general knowledge):

• Typically comprises carbon sources, nitrogen/peptone sources, mineral salts, and selective agents that inhibit non-target flora (literature).
• Selectivity for Lactobacillus is commonly achieved via low pH and selective inhibitors; surfactants or acetate sources may be present in some literature formulations.
• As a broth (non-solid), it does not contain agar; intended for liquid culture enrichment (literature).

2D/functional description in words:

• A powdered blend designed to yield an acidic, nutrient-rich aqueous medium after dissolution and sterilization, supporting lactic acid bacteria while suppressing competing microorganisms (literature).

Synthetic Utility

Not applicable. This product is a biological culture medium and is not used as a reagent or building block in chemical synthesis.

• For chemical synthesis needs (e.g., buffer reagents, catalysts, solvents), consult appropriate chemical product categories. For microbiology-focused work, refer instead to Application Protocols and Storage & Reconstitution sections in this listing.

Target Specificity

• Intended selectivity: Enrichment and cultivation of Lactobacillus spp. (general, literature).
• Mode of selectivity (literature): Combination of acidic pH and selective agents that inhibit many Gram-negative bacteria and non-target Gram-positives while allowing lactic acid bacteria to grow.
• Scope and limits: Selectivity is not absolute; some related lactic acid bacteria or acid-tolerant species may also grow. Conversely, particularly fastidious Lactobacillus strains might require supplementation or modified conditions.

Item-specific tested organisms, clone/epitope/isotype-style details: Not applicable; no antibody target data. No item-specific growth-promotion organism list is provided for this product; Not specified for this item; refer to CoA/Spec Sheet or perform in-house verification with reference strains.