LB Agar

Cat. No.: L1450101

Storage

Protected from light,Room temperature,Desiccated

Shipped In

Normal

Übersicht

LB Agar is a growth media for the cultivation of a wide variety of microorganisms.

Used for general bacterial culture, especially for the preservation and culture of Escherichia coli in molecular biology experiments.

Testing principle

Peptone and yeast extract powder provide nitrogen sources, vitamins, and growth factors; sodium chloride maintains a balanced osmotic pressure; glucose provides a carbon source; and agar is the coagulant for the culture medium.

Ingredients of LB agar medium formula

Formula (per liter)	Content
Peptone	10.0 g
Sodium chloride	5.0g
Glucose	1.0g
Yeast extract powder	5.0g
Agar	15.0g
Final pH	7.0±0.2

Instructions for use

Weigh 36g of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, then aliquot into test tubes or conical flasks, sterilize at 121℃ for 15 minutes, and set aside for later use.

LB agar medium quality control [inoculation of the following quality control strains into the test medium]:

Quality control strain and number	Indicator	Culture conditions	Quality control evaluation criteria
Escherichia coli ATCC25922	Growth rate	Cultured at 36℃±1℃ for 18~24h	PR≥0.7, grayish-white colonies
Escherichia coli ATCC8739

Spezifikationen

Storage

Protected from light,Room temperature,Desiccated

Verschickt in

Normal

Chemische und physikalische Eigenschaften

• Empfindlichkeit: moisture sensitive