Leupeptin Solution - BioReagent,1 mg/mL , CAS No.103476-89-7

CAS: 103476-89-7 Cat. No.: B1518359 Molecular Weight: 475.59 EC Number: 600-443-5 PubChem CID: 2733491

GRADE & PURITY BioReagent, 1 mg/mL

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Protein Extraction

Overview

Protease inhibitors can bind to specific groups in the active centers of protease molecules, reducing or eliminating enzyme activity without denaturing the proteins. This effectively inhibits protein degradation and maintains native protein-protein interactions. Common protease inhibitors include EDTA, E-64, NaVO₃, Bestatin, Leupeptin, Pepstatin A, Aprotinin, etc.

Product Description

The Leupeptin Solution (1 mg/mL) is primarily composed of leupeptin. It is EDTA-free and provided as a concentrated solution. Its working concentration ranges from 1 to 10 μg/mL, with a commonly used concentration of 3 to 5 μg/mL. It is suitable for protein extraction from mammalian tissues and cells, facilitating efficient obtainment of target proteins. The extracted products can be used in subsequent experiments such as Western Blot, Co-Immunoprecipitation (Co-IP), etc.

This reagent is for research use only. Not for use in diagnostic procedures or other purposes.

Procedure (For Reference Only)

1. Briefly centrifuge the tube before opening to spin down any liquid adhering to the walls or cap.

2. Add this product to the required volume of Lysis Buffer and dilute to the desired target working concentration (1–10 μg/mL, 3–5 μg/mL recommended) before use

Notes

1. This product is mildly irritating. Handle with care and avoid repeated freezing and thawing.

2. For your safety and health, please wear laboratory appropriate attire and disposable gloves while handling reagents.

3. Use the reagent as soon as possible after opening to ensure optimal performance.

Specifications

Specifications & Purity

BioReagent, 1 mg/mL

Stability And Storage

Store at -20℃ long term (18 months). Avoid freeze/thaw cycle. Store in the dark.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent

Names and Identifiers

• Isomeric SMILES: CC(C)C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C(N)N)C=O)NC(=O)C.CC(C)C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C(N)N)C=O)NC(=O)C.OS(=O)(=O)O
• PubChem CID: 2733491
• Molecular Weight: 475.59

Chemical and Physical Properties

• Sensitivity: Light-sensitive

References

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2. Yunhua Zhang, Jinyun Ji, Xiao Zhao, Ruoheng Jin, Jianhong Li, Hu Wan.  (2017)  Expression, purification and enzymatic properties of a β-N-acetylhexosaminidase from brown planthopper, Nilaparvata lugens.  JOURNAL OF ASIA-PACIFIC ENTOMOLOGY,      [PMID:] [10.1016/j.aspen.2017.10.005]

3. Rongrong He, Ziwei Lv, Yinan Li, Shuchao Ren, Jiaqi Cao, Jun Zhu, Xinrong Zhang, Huimin Wu, Lihao Wan, Ji Tang, Shutong Xu, Xiao-Lin Chen, Zhipeng Zhou.  (2024)  tRNA-m1A methylation controls the infection of Magnaporthe oryzae by supporting ergosterol biosynthesis.  DEVELOPMENTAL CELL,      [PMID:39191251] [10.1016/j.devcel.2024.08.002]

4. Bangbang Li, Yanchen Wang, Pengzhao Chang, Hao Chen, Yangang Zhu, Nanxin Zhao, Zhimou Yang, Jingjing Li.  (2025)  In situ enzyme instructed peptide assembly favoring a three-target sequentially responsive fluorescence probe for the early identification of atherosclerotic plaque in vivo.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.167162]