m-TGE Broth - BioReagent

Cat. No.: M1528381

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Übersicht

For determination of total colony counts in dairy products by membrane filtration

Specifications

Spezifikationen & Reinheit

BioReagent

Storage

Room temperature

Verschickt in

Normal

Note

BioReagent

Product Line

Biologische Produkte

Application Protocols

• General protocol examples (literature; adapt to your SOP and regulatory method):

1. Preparation of m-TGE Broth

• Add the manufacturer-recommended grams of dehydrated medium to 1 L of purified water (value not specified here; refer to CoA/label).
• Heat with stirring to dissolve completely; adjust volume for evaporation losses.
• Autoclave at 121 °C for 15 min. Cool to 45–50 °C. Aseptically dispense into sterile containers or saturate sterile absorbent pads for membrane work.

2. Membrane Filtration Enumeration

• Filter defined sample volumes through 0.45 µm sterile membranes using a validated manifold.
• Place membranes onto sterile pads saturated with m-TGE Broth or onto m-TGE Agar plates.
• Incubate inverted at the method-prescribed temperature (e.g., 30–37 °C) for 24–48 h.
• Count colonies and report CFU per volume filtered, applying dilution factors.

3. Growth Promotion Testing

• Inoculate prepared medium with low inocula of reference strains (e.g., E. coli, Enterococcus faecalis, Pseudomonas aeruginosa) as defined by your method.

• Incubate under specified conditions and verify expected recovery and morphology compared to control media.

• Item-specific tested applications, dilutions, or controls: Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

• Biological function (general, literature): m-TGE Broth is designed to support microbial metabolism rather than to act as a biochemical reagent. Its components typically serve the following roles:
  • Peptone/tryptone: Source of amino acids, small peptides, and nitrogen for biosynthesis and energy via deamination.
  • Glucose (dextrose): Primary carbon and energy source entering glycolysis and downstream respiratory/fermentative pathways.
  • Yeast/meat extract: Vitamins (B-complex), nucleotides, minerals, and growth factors enhancing biomass formation.
  • Phosphate or other buffers (if present): Maintain near-neutral pH during growth by neutralizing metabolic acids.
• Use context:
  • Supports growth of a broad range of non-fastidious, heterotrophic bacteria, facilitating colony development for enumeration and general cultivation.
• Note: Exact composition and concentrations for SKU M1528381 are Not specified for this item; refer to CoA/Spec Sheet. Performance characteristics (e.g., growth of specific strains) should be verified by growth promotion testing per your laboratory’s SOPs.

Buffer Applications

• Not typically used as a standalone buffering reagent. While prepared media may contain buffering salts to maintain near-neutral pH during microbial growth (literature), m-TGE Broth is not intended for analytical buffering, electrophoresis, or biochemical assay buffering.
• For buffering needs, employ defined buffers (e.g., phosphate, HEPES, Tris) prepared separately to the required pH and ionic strength.

Green Alternatives

• Environmental considerations (general):
  • Dehydrated media reduce shipping weight and packaging compared with ready-to-use liquids, lowering transportation footprint.
  • Preparing only the volume needed minimizes waste; autoclaving small batches in borosilicate bottles reduces single-use plastics.
• Comparative options (literature):
  • R2A media for low-nutrient waters can reduce incubator energy/time by enabling growth at lower temperatures over longer periods, potentially improving recovery from oligotrophic environments and reducing repeat testing.
  • Ready-to-use, irradiated media in recyclable PET bottles can decrease steam/energy use (no autoclave), at the expense of higher packaging waste.
• Example tradeoffs (general):
  • In-house autoclaving: higher energy/water use but minimal plastic waste and greater flexibility.
  • Pre-sterilized units: lower on-site energy but increased packaging and shipping mass.
• Greener practice tips:
  • Use energy-efficient autoclave cycles and load optimization.
  • Consolidate incubations, and employ timers/doors to limit heat loss.
  • Validate minimal effective incubation times/temperatures per method to reduce energy while preserving recovery.

Pharmaceutical Uses

• This product is intended for research use only (item-specific). It is not manufactured to GMP and is not indicated for clinical use.
• Relevant non-clinical/industrial contexts (literature):
  • Environmental monitoring and water testing in utilities, food & beverage, and non-sterile manufacturing settings (heterotrophic plate count via membrane filtration or pour-plate using corresponding agar formulations).
  • Growth promotion controls for sanitation verification programs.
• Pharmacopeial status: No monograph is implied for this product. If use in regulated QC is intended, verify method suitability and growth promotion per applicable compendia (e.g., USP, EP) and retain the lot-specific CoA.
• Excipient/formulation uses: Not applicable.

Physical Properties

• Physical state: Dehydrated culture medium (powdered blend) intended for aqueous reconstitution (general for media).
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Freely soluble in water upon heating and mixing (literature, typical for dehydrated broths).
• pH of prepared medium (literature): m-TGE formulations are commonly adjusted to approximately pH 7.0–7.3 at 25 °C after sterilization; exact value varies by manufacturer/specification.
• Osmolality/ionic strength: Varies with formulation (literature). Not specified for this item; refer to CoA/Spec Sheet.
• Preparation temperature (literature): Dissolves in purified water with gentle heat; sterilization typically by autoclaving at 121 °C. See Reaction Conditions/Protocols for general guidance.
• BP/MP, density, refractive index, logP, pKa: Not applicable or not meaningful for an undefined dehydrated mixture; not determined.
• Notes:
  • Because this is a complex blend, batch-to-batch specifications (e.g., pH of a 2% solution, clarity after autoclave) are defined on the product specification. For SKU M1528381, these values are Not specified for this item; refer to CoA/Spec Sheet.

Quality and Grades

• Grade: BioReagent (item-specific, per Product Data).
  • BioReagent grade generally indicates suitability for biological and biochemical laboratory workflows, with controlled bioburden and impurity profiles compatible with cell culture/microbiology (not necessarily sterile). It does not imply pharmaceutical GMP compliance.
• Typical quality attributes for dehydrated broth (general/literature): clarity after sterilization, pH of prepared medium, growth performance with control strains, and moisture content. These are defined per lot on a Certificate of Analysis.
• Stabilizers/additives: Not specified for this item; refer to CoA/Spec Sheet.
• UV cutoff, trace metals, peroxide, water content: Not specified for this item; refer to CoA/Spec Sheet.
• What this means for users:
  • Expect consistent growth performance when prepared according to recommended procedures.
  • Perform incoming QC as required by your QA system (e.g., pH check post-autoclave, growth promotion testing with reference organisms).
  • For regulated testing, confirm that the performance characteristics meet your method requirements and retain the lot-specific CoA.

Reaction and Applications

• This section is adapted to microbiological applications (no chemical reactions involved).
• Typical applications (literature):
  • Enumeration and cultivation of heterotrophic bacteria from water and environmental samples, frequently in membrane filtration methods; the “m-” designation reflects suitability for membrane-based workflows.
  • General-purpose enrichment/cultivation of non-fastidious aerobic and facultative anaerobic bacteria.
  • Growth promotion testing and sanitation monitoring in industrial microbiology (e.g., beverage, bottled water, utilities), where a glucose/tryptone/extract nutrient base is appropriate.
• Rationale:
  • Tryptone/peptone provides peptides and amino acids; glucose supplies a readily metabolizable carbon source; extract components furnish vitamins and growth factors, yielding robust colony development.
• Practical tips (literature):
  • Ensure complete dissolution before sterilization to avoid precipitates; adjust volume to compensate for evaporation during autoclaving.
  • For membrane filtration, impregnate sterile absorbent pads with sterile m-TGE broth or pour plates with corresponding agar medium; incubate inverted to limit condensation.
  • Include suitable negative/positive controls; if sample contains residual disinfectants, incorporate neutralizers as appropriate per method.

Reaction Conditions

• Interpreted here as preparation and cultivation conditions (literature/general guidance). Exact conditions for SKU M1528381 are Not specified for this item; refer to CoA/Spec Sheet.
• Typical preparation (literature):
  • Dissolve the specified mass of dehydrated m-TGE Broth per liter of purified water (manufacturer formula dependent; follow label/CoA). Heat with agitation until fully dissolved.
  • Adjust pH if directed by specification (commonly near 7.1 ± 0.2 at 25 °C after sterilization for similar media).
  • Sterilize by autoclaving at 121 °C for 15 minutes. Allow to cool to 45–50 °C before dispensing aseptically.
• Membrane filtration workflow (literature):
  • Filter measured sample volumes through 0.45 µm sterile membranes.
  • Place membranes onto sterile absorbent pads saturated with sterile m-TGE Broth or onto m-TGE Agar.
  • Incubate plates inverted at method-specified temperature/time (commonly 30–37 °C, 24–48 h depending on standard and matrix). Enumerate colonies.
• Notes:
  • Optimize conditions per applicable standard methods and SOPs. Include positive/negative controls and, if needed, neutralizers for residual disinfectants.

Safety and Handling

• GHS classification, signal word, H/P statements, pictograms: Not specified for this item; refer to SDS.
• General hazards (literature/best practice):
  • Low acute toxicity expected for typical nutrient media components, but handle as laboratory chemical. Avoid inhalation of dust and contact with eyes/skin.
  • Use appropriate PPE: lab coat, gloves, safety glasses; wear a dust mask or work in a hood if generating dust.
  • After reconstitution/sterilization and use with microorganisms, treat as biohazardous waste; autoclave before disposal per institutional biosafety protocols.
• Incompatibilities: Avoid strong oxidizers and reactive chemicals that could degrade peptides/sugars (general). Store dry to prevent caking and microbial contamination.
• First aid (overview; defer to SDS):
  • Inhalation: Move to fresh air; seek medical advice if irritation persists.
  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; seek medical attention if irritation continues.
  • Ingestion: Rinse mouth; do not induce vomiting; obtain medical attention if unwell.
• Fire safety: Organic powder; may burn if involved in a fire. Use water spray, CO2, dry chemical, or foam. Avoid dust clouds.
• Always consult the product SDS for authoritative hazard and response information.

Solvent Selection

• Applicability: This product is a dehydrated culture medium and is intended to be reconstituted in purified water. It is not used as a solvent in chemical synthesis.
• Reconstitution solvent (general guidance):
  • Use high-purity water (e.g., Type I/II ASTM, or equivalent) free from inhibitory residuals (chlorine, ozone). For membrane filtration methods, sterile buffered diluents are commonly used downstream.
• Miscibility/polarity: Upon reconstitution, the broth is an aqueous medium; organic co-solvents are generally not used as they can inhibit microbial growth.
• When to choose m-TGE vs alternatives (literature):
  • m-TGE is favored for heterotrophic bacterial counts, especially in membrane-filtration workflows, due to its balanced nutrients and clarity.
  • Alternatives include R2A (for oligotrophic water samples), Plate Count Broth/Agar (standard HPC), or nutrient broth (general cultivation). Selection depends on sample matrix and target bioburden.

Storage and Reconstitution

• Storage (item-specific): Room temperature (per Product Data). Keep tightly closed in a dry place. Protect from moisture to prevent caking and inadvertent microbial growth.
• Shipping: Normal (per Product Data).
• Shelf life, moisture limits: Not specified for this item; refer to CoA/Spec Sheet.
• Reconstitution (general, literature):
  • Reconstitute in purified water at the manufacturer-recommended concentration. Mix with gentle heat until dissolved. Adjust pH only if directed by the specification.
  • Sterilize by autoclaving at 121 °C for 15 minutes unless using sterile, pre-filtered single-use formats.
  • For membrane filtration, aseptically saturate sterile absorbent pads with the sterile broth; avoid over-wetting.
• Storage after preparation (general):
  • Store sterile broth at 2–8 °C protected from light. Use as soon as practical; typical in-use stability is limited (days to weeks) and should be established by the user.
  • Avoid repeated heating/cooling cycles that can alter performance.
• Research use note: For research use only (per Product Data).

Structure and Identity

• Item type: Complex, dehydrated microbiological culture medium (broth); not a single defined chemical entity.
• Molecular formula: Not applicable to mixtures.
• Molecular weight: Not applicable to mixtures.
• SMILES / InChI / InChIKey: Not applicable to mixtures of undefined composition.
• Item-specific identifiers (CAS, CID): Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (general, literature): m-TGE (modified Tryptone–Glucose–Extract) media are typically composed of a peptone/tryptone digest (amino acids/peptides), a carbohydrate source (glucose/dextrose), and complex growth factors (e.g., yeast extract or meat extract), sometimes with phosphate for buffering. These components provide nitrogen, carbon, vitamins, and minerals suitable for heterotrophic bacterial growth.
• 2D description (general): As a mixture, it contains small peptides and amino acids (amide linkages), simple sugars (polyhydroxy aldehyde/ketone derivatives), and a distribution of vitamins and nucleotides from extract components. No single discrete molecular structure applies.
• Note: Exact composition, concentrations, and any supplements for SKU M1528381 are Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

• Not applicable. m-TGE Broth is a biological culture medium and is not used as a reagent or intermediate in chemical synthesis.
• For chemical synthesis planning and solvent selection, refer to reagents/solvents specifically designed for those purposes.

Target Specificity

• Not applicable. This product is not an antibody, probe, or inhibitor with molecular target specificity. It is a non-selective culture medium intended to support growth of a broad range of heterotrophic bacteria (literature).