M17 Broth - BioReagent

Cat. No.: M1528724

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Übersicht

For detection of lactic acid bacteria in milk and dairy products

Specifications

Spezifikationen & Reinheit

BioReagent

Storage

Room temperature

Verschickt in

Normal

Note

BioReagent

Product Line

Biologische Produkte

Application Protocols

No item-specific tested application protocols are provided in the Product Data. The following general protocol reflects common practice for M17-type media (literature/general). Adjust per your SOP and consult the CoA/Spec Sheet for any item-specific instructions.

General broth preparation:

• Weigh the required mass of dehydrated medium for the desired final volume (use supplier-recommended g/L; item-specific value Not specified here).
• Add to ~90% of the final volume of high-purity water with stirring until fully dissolved. Adjust pH if required.
• Sterilize by autoclaving at 121 °C for 15 minutes (if heat-stable). Cool to 45–55 °C. Aseptically add sterile-filtered heat-labile supplements if needed. Bring to final volume with sterile water.
• For plates: Add sterile agar (typically 1.5–2% w/v; literature) before sterilization or as a sterile molten solution; pour plates in a laminar-flow hood.

Inoculation and incubation:

• Inoculate with a pure culture. Incubate at organism-appropriate temperature (commonly 30–42 °C for LAB; literature). Monitor growth by OD600 or viable counts.

Quality control:

• Include uninoculated negative controls. Perform growth promotion testing with reference strains when qualifying new lots.

Biological Roles

This product itself has no intrinsic biological role; it is an enabling medium that supplies carbon, nitrogen, minerals, vitamins, and buffering capacity to support microbial metabolism.

Context for M17-type media (literature/general):

• Target organisms: Optimized for growth of lactic acid bacteria (LAB), particularly Lactococcus spp. and Streptococcus thermophilus, which are homofermentative and produce lactic acid as the primary end product of carbohydrate metabolism.
• Metabolic support: Complex peptides and amino acids relieve biosynthetic burdens; carbohydrates (e.g., lactose or glucose) fuel glycolysis; phosphate/glycerophosphate buffers moderate acidification to sustain growth phases.
• Physiological studies: Suitable for probing sugar uptake systems (PTS vs. permease), acid tolerance responses, bacteriophage interactions, and bacteriocin production, depending on supplements added (literature).
• Growth characteristics: LAB often require rich media with growth factors (B-vitamins from yeast extract) and careful control of redox/pH; M17-type media address these needs.

Item-specific details such as the exact nutrient profile, buffering species, and carbohydrate identity are Not specified for this item; refer to CoA/Spec Sheet. Adjust incubation temperature, atmosphere, and agitation to the organism’s requirements and experimental objectives.

Buffer Applications

This product is a complete culture medium, not a dedicated buffering reagent. However, many M17-type formulations incorporate buffering components that stabilize pH during lactic fermentation.

• Intended use: Growth and maintenance of LAB rather than preparation of analytical buffers.
• pH range (prepared medium): Literature reports near-neutral initial pH (typically ~6.8–7.0) for M17-type broths; exact target for this item is Not specified; refer to CoA/Spec Sheet.
• Practical notes (literature/general):
  • If a defined buffer is required for downstream assays, cells can be harvested from M17 broth and washed/resuspended in a dedicated buffer (e.g., phosphate, MOPS) of known composition.
  • For strong acid producers, additional buffering or controlled neutralization (e.g., CaCO3 addition in solid media) may be employed; validate compatibility with your organism.

Because composition is not provided for this SKU, do not use this product as a reference buffer. For electrophoresis, chromatography, or enzyme assays, prepare standard, well-characterized buffers instead.

Green Alternatives

Environmental considerations for culture media focus on water/energy use, waste minimization, and sourcing rather than solvent replacement.

Sustainability strategies (literature/general):

• Energy-efficient sterilization: Consolidate batches to optimize autoclave loading; use validated minimum sterilization cycles. Where feasible, sterile filtration of heat-labile supplements reduces energy consumption versus multiple autoclave runs.
• Waste reduction: Prepare only volumes required for the near term. Autoclave spent media and cultures before disposal; consider on-site treatment systems that reduce biohazard volume.
• Component sourcing: Complex nitrogen sources (peptones/yeast extracts) vary in environmental footprint. When possible, select formulations with responsibly sourced raw materials (consult supplier documentation).
• Packaging: Choose pack sizes that minimize repeated opening (moisture ingress) and reduce plastic waste. Reuse/repurpose secondary packaging where permitted by QA.

Alternatives within media selection (literature/general comparison):

• For lactic acid bacteria, other media (e.g., MRS broth) may offer similar performance. Selection should be based on organism-specific growth, pH control, and assay needs rather than “greenness” alone. Validate performance equivalency before substitution to avoid rework/waste.

Note: No item-specific green certifications or composition disclosures are provided here; refer to CoA/Spec Sheet for any available sustainability statements.

Pharmaceutical Uses

No therapeutic or clinical diagnostic uses are claimed. For research use only.

Relevant roles in pharmaceutical/biotech laboratories (literature/general):

• QC microbiology: Cultivation and enumeration of reference strains in environmental monitoring or growth promotion testing (per pharmacopeial guidance), after appropriate suitability verification.
• Process development: Seed-train cultivation of lactic acid bacteria for exploratory fermentation studies, strain banking, and physiological characterization.
• Raw material qualification: A medium for assessing nutrient requirements, antibiotic susceptibility, or product-induced growth inhibition during early development.

Compliance and documentation:

• This SKU is designated BioReagent grade; it is not certified to any pharmacopeial monograph based on the provided data. Any GMP or QC use requires internal qualification, method validation, and lot-specific CoA/Spec documentation.
• If using in regulated settings, establish acceptance criteria for pH, clarity, and growth promotion with relevant compendial organisms and document results per your QMS.

Item-specific compendial status, sterility assurances, or traceability details are Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific physicochemical specifications are not provided in the product data for this complex medium.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Freely soluble in purified water (general for dehydrated culture media; literature). Final clarity may range from clear to slightly opalescent after sterilization depending on formulation (literature/general).
• pH (prepared medium): Typical M17 formulations yield pH ~6.8–7.0 at 25 °C before sterilization (literature). Not specified for this item; refer to CoA/Spec Sheet for lot-specific pH targets.
• Density, refractive index, BP/MP, logP, pKa: Not applicable or not defined for mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• Hygroscopicity: Dehydrated microbiological media are generally hygroscopic; protect from moisture (general guidance).

Preparation factors (literature/general):

• Typical working concentration and carbohydrate choice vary (e.g., lactose- or glucose-containing variants). Heat treatment may alter clarity/color due to Maillard reactions. Degassing prior to sterilization can reduce foaming. Filter or autoclave sterilization practices depend on heat-labile components present. Always verify exact preparation instructions on the CoA/Spec Sheet for this SKU.

Quality and Grades

• Grade: BioReagent (as provided in Product Data). This denotes suitability for laboratory biological applications where consistent performance in culture workflows is required. It does not imply clinical/diagnostic use.

What BioReagent grade generally entails (literature/general):

• Raw material controls intended to minimize lot-to-lot variability in growth performance, pH, and clarity.
• Microbiological quality checks (e.g., growth promotion testing with representative organisms) may be part of release criteria; consult CoA for organisms and acceptance ranges if applicable.
• Lower levels of inhibitory contaminants (e.g., heavy metals, antimicrobial residues) relative to commodity-grade components, though specific numeric limits are not stated here.

Item-specific notes:

• Specific composition, performance claims, and any additives (e.g., selective agents, carbohydrate source) are Not specified for this item; refer to CoA/Spec Sheet.
• UV cutoff, ash content, moisture limits, and trace analyses are Not specified for this item; refer to CoA/Spec Sheet if needed for regulated workflows.

Use guidance:

• For reproducible results, use high-purity water (e.g., ASTM Type 1/2), calibrated balances, and validated sterilization. Retain CoA/Spec Sheet with the batch record to document performance characteristics in your SOPs.

Reaction and Applications

As a microbiological growth medium, M17 Broth is not used as a chemical reagent in synthetic reactions. Its applications are biological and process-focused.

Representative research applications (literature/general for M17-type media):

• Cultivation and enumeration of lactic acid bacteria, notably Lactococcus spp. and Streptococcus thermophilus.
• Dairy microbiology workflows, including starter culture maintenance and phage-monitoring assays.
• Biotechnology seed trains for lactic fermentations (e.g., lactic acid, flavor compounds), where robust growth and buffering are required.
• Physiological studies of carbohydrate utilization, acid tolerance, and bacteriocin production when paired with defined carbohydrate sources.

Practical tips:

• Carbohydrate source matters: lactose vs. glucose can shift growth kinetics and acidification (literature). Match the carbohydrate to your organism and experimental goals.
• Buffering: M17-type media often include glycerophosphate/phosphate buffers that mitigate pH drops during lactic acid production (literature). Verify buffer system and target pH on the CoA/Spec Sheet.
• Supplements: For selective/enhanced growth, aseptically add antibiotics, indicators, or additional nutrients after sterilization, observing compatibility and sterility.
• Quality control: Perform growth promotion testing with lab strains relevant to your workflow to qualify each lot for critical experiments.

Reaction Conditions

Chemical reaction conditions are not applicable. Instead, below are general culture preparation and incubation conditions for M17-type media (literature/general). Item-specific instructions are Not specified for this product; refer to CoA/Spec Sheet.

Preparation (general):

• Dissolve the dehydrated medium in high-purity water at the supplier’s specified concentration (literature examples for M17 are often in the 40–55 g/L range). Adjust pH to near neutral if required.
• Sterilization: Autoclave at 121 °C for 15 minutes if fully heat-stable. If heat-labile supplements are used, sterilize them by filtration and add aseptically to cooled (45–55 °C) sterile medium.

Incubation (organism-dependent):

• Typical targets for LAB: 30–42 °C, static or gentle agitation, aerobic to microaerophilic conditions depending on species/strain (literature).
• Monitor OD600, viable counts, and pH. LAB acidify the medium; buffering capacity limits rapid pH drops, but neutralization strategies may be required for extended cultures.

Quality checks:

• Clarity and color post-sterilization; absence of contamination in uninoculated controls.
• Growth promotion with a lab reference strain relevant to your workflow.

Always validate conditions for your specific organism and experimental aims.

Safety and Handling

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to the product SDS for authoritative hazard information.
• General hazards (literature/general for dehydrated media): Low acute hazard expected. Dust may cause mechanical irritation to eyes, skin, and respiratory tract. May form dust clouds; avoid inhalation and ignition sources.
• PPE: Laboratory coat, appropriate gloves (e.g., nitrile), safety glasses/goggles. Dust mask/respirator (P2/N95) if airborne particulates may be generated.
• Handling: Minimize dust generation. Use in well-ventilated area or fume hood when weighing. Avoid moisture pickup; reseal promptly.
• Incompatibilities: Strong oxidizers and strong acids/bases may degrade organic constituents (general). Avoid contact with moisture until use.
• First aid (general):
  • Inhalation: Move to fresh air; seek medical attention if symptoms persist.
  • Skin: Wash with soap and water; remove contaminated clothing.
  • Eyes: Rinse cautiously with water for several minutes; remove contact lenses if present and easy to do; continue rinsing.
  • Ingestion: Rinse mouth; seek medical advice if unwell.
• Microbiological safety: Prepared broth becomes biologically active once inoculated. Handle cultures using appropriate biosafety practices (e.g., BSL-1/2 as applicable), disinfect work surfaces, and autoclave waste.
• SDS: Always consult the current SDS for SKU M1528724 before use.

Solvent Selection

This product is a dehydrated culture medium intended to be dissolved in water; organic solvent selection is not applicable.

Practical guidance (literature/general):

• Solvent: Use freshly prepared, high-purity water (ASTM Type 1/2 or equivalent). Avoid chlorinated tap water; residual oxidants can inhibit growth.
• Sterility: Prepare under aseptic conditions. Sterilize by autoclaving if the formulation is fully heat-stable; alternatively, sterile-filter heat-labile supplements and add aseptically post-sterilization.
• Dissolution: Add medium gradually to stirred water to minimize clumping and foaming. Gentle warming (<60 °C) can aid dissolution when appropriate.
• Deaeration: For microaerophilic/anaerobic work, pre-reduce and deoxygenate the prepared broth (e.g., sparging with N2/CO2 or incubation in an anaerobic chamber) as required by the organism.
• pH adjustment: Verify and adjust pH before sterilization per your SOP. Typical literature values for M17-type media are near neutral; confirm item-specific targets on the CoA/Spec Sheet.

Storage and Reconstitution

• Storage conditions (as provided): Room temperature. Protect from moisture, heat, and direct sunlight. Keep container tightly closed. Shipped under normal conditions.
• Shelf life: Not specified for this item; refer to CoA/Spec Sheet. Use by the labeled expiration or best-before date if provided.

Reconstitution and sterilization (literature/general for M17-type media):

• Dissolve at the supplier-recommended concentration in high-purity water. Item-specific g/L and target pH are Not specified for this item; refer to CoA/Spec Sheet.
• Mix thoroughly to dissolve; gentle warming can assist. Adjust pH if required by your SOP.
• Sterilize by autoclaving at 121 °C for 15 minutes if all components are heat-stable. Alternatively, sterile-filter heat-labile supplements and add aseptically to cooled sterile base medium.
• Storage of prepared medium: Store at 2–8 °C, protected from light. Use promptly (typical practice: within days to a few weeks depending on sterility and composition; literature). Discard if turbidity or precipitate indicative of contamination appears.
• Freeze–thaw: Avoid freezing prepared complex media; repeated freeze–thaw can cause precipitation and performance drift.

Always consult and follow the CoA/Spec Sheet for SKU M1528724 for any item-specific preparation parameters and stability guidance.

Structure and Identity

This product is a complex, multi‑component microbiological medium (M17 Broth) supplied as a dehydrated blend; it is not a single defined chemical entity.

• Product type: Complex culture medium for lactic acid bacteria (research/lab use)
• Molecular formula: Not applicable (mixture); Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not applicable (mixture); Not specified for this item; refer to CoA/Spec Sheet.
• CAS, CID, InChIKey, SMILES: Not applicable to complex media; Not specified for this item; refer to CoA/Spec Sheet.
• Structural features: Heterogeneous blend typically comprising peptones/tryptone, yeast/beef extracts, carbohydrate(s), salts, and buffering components (literature, general for M17-type media).
• 2D structural description: Not applicable; mixture of peptides, amino acids, carbohydrates, minerals, and vitamins without a single defined molecular structure.

Notes on identity (literature/general):

• “M17” media are widely described for cultivation and enumeration of Lactococcus spp. and Streptococcus thermophilus. Typical formulations include complex nitrogen sources plus a phosphate/glycerophosphate buffer and a carbohydrate (often lactose or glucose). Exact composition and concentrations vary by supplier and lot; always consult the specific CoA/Spec Sheet for this SKU (M1528724).

Synthetic Utility

Not applicable as a chemical building block. M17 Broth is a complex culture medium and is not employed in organic synthesis or as a reagent/catalyst.

Related laboratory utility (literature/general):

• Biological production: Supports biomass generation of lactic acid bacteria for biotransformations, enzyme preparation, or metabolite production studies.
• Sample preparation: Source medium for conditioned supernatants used in antimicrobial assays or quorum-sensing investigations (after appropriate sterilization/clarification).

For synthetic chemistry workflows, select defined reagents and solvents designed for reaction compatibility and purity control. Use this product only within microbiology/bioprocess contexts.

Target Specificity

Not applicable. This product is a culture medium and does not target a biomolecular epitope or receptor. No antibody/oligonucleotide/probe specificity data apply.