Mob1A Antibody - Primärer Antikörper, specific to Mob1A, Rabbit IgG

Cat. No.: Ab115772

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.4 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human Mob1A Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

• WB: 1/500-1/1000
• IHC: 1/50-1/100 

Protein Function

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L.

Specifications

Product Name

Mob1A Antibody - Primärer Antikörper, specific to Mob1A, Rabbit IgG

Synonyme

B1, Mps One Binder kinase activator like 1B (yeast) antibody | C2orf6 antibody | Chromosome 2 open reading frame 6 antibody | FLJ10788 antibody | FLJ11595 antibody | MATS1 antibody | MOB kinase activator 1A antibody | MOB1 antibody | Mob1 alpha antibody |

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 0.4 mg/mL

Wirtsspezies

Kaninchen

Spezifität

Mob1A

Immunogen

A synthetic peptide of human MOB1A (AA 5-33)

Positive Kontrolle

WB: MOLT-4, Jurkat, K562, A375 and C6 cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

0.4 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Mob1A Antibody (Ab115772)

Mob1A Antibody (Ab115772) - Western Blot
All lanes: Mob1A Antibody (Ab115772) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 17, 25 kDa
Exposure time: 25.2 s

IHC: Mob1A Antibody (Ab115772)

Mob1A Antibody (Ab115772) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using Mob1A Antibody (Ab115772). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Mob1A Antibody (Ab115772)

Mob1A Antibody (Ab115772) - IF/ICC

IF analysis of Mob1A Antibody (Ab115772) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Mob1A Antibody (Ab115772) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).  

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Zugehörige Ziele (menschlich)

MOB1A Tbio MOB kinase activator 1A (0 Activities)

Discover Target: MOB1A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Mob1A Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.