Monensin Solution (1,000X) - sterile-filtered,BioReagent,1000X

Cat. No.: M1456520

GRADE & PURITY Sterile-filtered, BioReagent, 1000X

Synonyms

Monensin Sodium

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Flow Cytometry

Übersicht

  Monensin is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation.       Especially useful for the intracellular staining of cytokines, monensin leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum . Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest.

  This monensin is in the sodium salt form, which is supplied as a 1000X working solution in 70% Ethanol.

Usage Protocol

  Dilute the 1000X solution to 1X in the cell culture medium. 

Precautions

  It is recommended that cells are cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.

Specifications

Synonyme

Monensin Sodium

Spezifikationen & Reinheit

sterile-filtered,BioReagent,1000X

Stabilität und Lagerung

Store at -20℃ long term (12 months) or store at 4℃ short term (6 months).

Storage

Store at -20°C

Verschickt in

Ice chest + Ice pads

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Note

BioReagent, Steril gefiltert

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  1/1000;

Häufig gestellte Fragen

What does sterile-filtered mean?

sterile-filtered indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.