Mouse Fc Receptor Blocking Solution Plus - sterile-filtered, BioReagent, 10 μL/test

Cat. No.: M1374079

GRADE & PURITY Sterile-filtered, BioReagent, 10 μL/test

Synonyms

Cyanine dye blocking solution | Blocking buffer for Flow | Blocking Reagent | Fc Receptor Blocker | FcR Blocking Reagent | Fc Receptor Blocking Solution

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Application

Flow Cytometry

Übersicht

Non-specific binding to Leukocytes is a well-known challenge in flow cytometry. Though the specific mechanism of non-specific binding is not known, the binding of cyanine acceptor fluorophores to CD64 has been implicated in the literature. Mouse Fc Receptor Blocking Solution Plus not only specifically blocks non-specific antibody binding to CD16/CD32 (low-affinity IgG Fc receptors III and II), preventing non-specific binding of immunoglobulins to Fc receptors, but also effectively inhibits non-specific staining by various PE, PerCP, and APC tandem dyes on leukocytes. Compared to using anti-mouse CD16/32 mouse Fc receptor blocker alone, the new product demonstrates superior blocking performance against non-specific signals on Cyanine dyes.

This buffer contains specialized Rat IgG containing 0.05% ProClin 300. It is not recommended to be used for staining Rat IgG. Handle as biohazard agent.

Mouse Fc Receptor Blocking Solution Plus blocks both clone P90A23 and 2.4G2 also raised against mouse CD16/32.

Specifications

Synonyme

Cyanine dye blocking solution | Blocking buffer for Flow | Blocking Reagent | Fc Receptor Blocker | FcR Blocking Reagent | Fc Receptor Blocking Solution

Spezifikationen & Reinheit

sterile-filtered, BioReagent, 10 μL/test

Stabilität und Lagerung

Store at 2-8°C for 12 months. When stored at 2°C to 8°C, the presence of serum in this product may cause aggregation of various proteins and lipoproteins (such as cold agglutinins, fibrinogen, vitronectin, etc.), forming visible precipitate or cloudiness.

Storage

Store at 2-8°C,Protected from light

Verschickt in

Wet ice

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Note

BioReagent, Steril gefiltert

Bilder

Flow Cytometry: Mouse Fc Receptor Blocking Solution Plus (M1374079)

Mouse Fc Receptor Blocking Solution Plus (M1374079) - Flow Cytometry
Mouse Fc Receptor Blocking Solution Plus (M1374079) reduces nonspecific staining but preserves specific staining of mouse bone marrow cells (BMC) for CD11b. Mouse BMC were either not preincubated (Left Plots) or were preincubated (Middle Plots) with Mouse FcR Blocking Reagent (Ab219990), or were preincubated (Right Plots) with Mouse Fc Receptor Blocking Solution Plus as indicated. The cells were then stained with Rat IgG (PE-Cy5) (Ab190072) and recombinant CD11b Antibody (AF405) (Ab213132). Two-dimensional pseudocolor plots of CD11b vs Rat IgG show that the Mouse Fc Receptor Blocking Solution Plus reduces non-specific binding compared to staining without any blocking reagent and with Mouse FcR Blocking Reagent.

Flow Cytometry: Mouse Fc Receptor Blocking Solution Plus (M1374079)

Mouse Fc Receptor Blocking Solution Plus (M1374079) - Flow Cytometry
Mouse Fc Receptor Blocking Solution Plus (M1374079) reduces nonspecific staining but preserves specific staining of mouse splenocytes for CD45R. Mouse splenocytes were either not preincubated (Left Plots) or were preincubated (Middle Plots) with Mouse FcR Blocking Reagent (Ab219990), or were preincubated (Right Plots) with Mouse Fc Receptor Blocking Solution Plus as indicated. The cells were then stained with Rat IgG (PE-Cy5) (Ab190072) and CD45R Rat mAb (FITC) (Ab191667). Two-dimensional pseudocolor plots of CD45R vs Rat IgG show that the Mouse Fc Receptor Blocking Solution Plus reduces non-specific binding compared to staining without any blocking reagent and with Mouse FcR Blocking Reagent.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  10 μL/test. ; It is recommended to add 10 µL of Mouse Fc Receptor Blocking Solution Plus pe

Häufig gestellte Fragen

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

What does sterile-filtered mean?

sterile-filtered indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.