mTOR inhibitor-8 - 10mM in DMSO , CAS No.2489196-70-3

CAS: 2489196-70-3 Cat. No.: M656008 Summenformel: C₂₄H₁₉ClN₄OS Molekulargewicht: 446.95

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Übersicht

mTOR inhibitor-8 is an mTOR inhibitor and autophagy inducer. mTOR inhibitor-8 inhibits the activity of mTOR via FKBP12 and induces autophagy of A549 human lung cancer cells

In Vitro

mTOR inhibitor-8 (Compound 5e; 0.1-10 μM; 24 and 48 hours) suppresses the growth of A549 cells in a dose-dependent manner. mTOR inhibitor-8 induces autophagy in an mTOR-dependent manner. mTOR inhibitor-8 (10 μM; 3-24 hours) induces autophagy in a time-dependent manner. The levels of LC3B-II are enhanced. mTOR inhibitor-8 (10 μM; 3-24 hours) reduces the phosphorylation of RPS6KB1 (ribosomal protein S6 kinase) and EIF4EBP1 (eukaryotic translation initiation factor 4E-binding protein 1), two essential substrates of mTORMCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: A549 cells Concentration: 0.1, 1, 5, 10 μM Incubation Time: 24 and 48 hours Result: Suppressed the growth of A549 cells with an IC 50 of 2.6±0.11 μM. Western Blot AnalysisCell Line: A549 cells Concentration: 10 μM Incubation Time: 3, 6, 12 and 24 hours Result: The levels of phosphorylation of RPS6KB1 and EIF4EBP1 were significantly decreased after treatment.

In Vivo

mTOR inhibitor-8 (25 and 50 μM; 6 days) effectively inhibits tumor growth in vivo without adverse effect on normal chick chorioallantoic membrane angiogenesis . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Fertile chicken eggs (7-9 days old) Dosage: 25 and 50 μM Administration: 6 days Result: Significant xenograft tumor remission was observed in eggs compared with the DMSO-treated eggs.

IC50& Target:mTOR Autophagy

Specifications

Spezifikationen & Reinheit

10mM in DMSO

Biochemische und physiologische Mechanismen

mTOR inhibitor-8 is an mTOR inhibitor and autophagy inducer. mTOR inhibitor-8 inhibits the activity of mTOR via FKBP12 and induces autophagy of A549 human lung cancer cells.

Storage

Store at -80°C

Verschickt in

Dry ice packs + Cold packs

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Namen und Kennungen

Molekulargewicht

446.95

Häufig gestellte Fragen

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2489196-70-3, the molecular formula is C24H19ClN4OS, and the molecular weight is 446.95 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.