Mucinase StcE - Recombinant, mass spec suitable, from EHEC, powder

Cat. No.: M755176

GRADE & PURITY Recombinant, mass spec suitable, from EHEC, powder

Synonyms

Mucin-specific protease StcE, secreted protease of C1 esterase inhibitor

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Übersicht

Mucinase StcE is a metalloprotease that can be used to digest densely O-glycosylated mucins. The consensus sequence for cleavage is S/T*-X-S/T, where the cleavage happens on the amino side of the 2^nd Serine/Threonine, and X can be any amino acid, or none though with lower efficiency. The P2 position (*) is found to be always occupied in cleavage products, and occupancy of both P1 and P1’ positions do not interfere with cleavage. While Mucinase StcE is active against Mucins and other mucin-domain containing glycoproteins such as CD43 or PSGL-1, it is not active against O-glycoproteins outside of the Mucin family, such as fetuin or GspB736flag¹.

Application:

For use as a tool in biochemical research and biomarker discovery, particularly focused on glycobiology, glycoproteomics, glyco-oncology. Shown to be useful in LC-MS/MS workflows

Specifications

Synonyme

Mucin-specific protease StcE, secreted protease of C1 esterase inhibitor

Spezifikationen & Reinheit

Recombinant, mass spec suitable, from EHEC, powder

Biochemische und physiologische Mechanismen

Mucins are heavily O-glycosylated proteins found in many tissues such as mucosal membranes and intestinal linings. One of its functions is as a protective layer for the epithelial tissue. The pathogenic bacteria enterohemorrhagic Escherichia coli (EHEC)

Stabilität und Lagerung

The product retains activity for at least 2 years when stored lyophilized at -20°C

Storage

Store at -20°C

Verschickt in

Ice chest + Ice pads

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Note

Recombinant

Häufig gestellte Fragen

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.