MX1 Antibody - Primärer Antikörper, specific to MX1

Cat. No.: Ab116582

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, See COA

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Conjugation

Unconjugated

Übersicht

Rabbit anti Human MX1 Antibody, Polyclonal, could be used for WB, IHC and so on.

Application:

WB: 0.5-5µg/mL

IHC: 5-20µg/mL

IF/ICC:  2µg/mL

Protein Function:

May regulate the calcium channel activity of TRPCs. Shows activity against influenza virus and VSV, a rhabdovirus. 

Specifications

Product Name

MX1 Antibody - Primärer Antikörper, specific to MX1

Synonyme

IFI-78K | Interferon-induced protein p78 | Interferon-regulated resistance GTP-binding protein MxA | Myxoma resistance protein 1 | Myxovirus resistance protein 1 | human interferon-regulated resistance GTP-binding protein MXA10IFI-78Khomolog of murine (in

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Azide Free, Validated, See COA

Wirtsspezies

Kaninchen

Spezifität

MX1

Immunogen

Recombinant MX1 expressed in E.coli (AA 80-342)

Positive Kontrolle

WB: Mouse lung, Mouse spleen, HeLa, A549 and C6 lysates. IHC: Human skin cancer, liver, breast cancer tissues. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Antigen affinity purified + Protein A purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - Western Blot
All lanes: MX1 Antibody (Ab116582) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 76 kDa
Observed band size: 78 kDa
Exposure time: 4.6 s

IHC: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL MX1 Antibody (Ab116582)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL MX1 Antibody (Ab116582)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL MX1 Antibody (Ab116582)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: MX1 Antibody (Ab116582)

MX1 Antibody (Ab116582) - IF/ICC  

IF analysis of MX1 Antibody (Ab116582) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MX1 Antibody (Ab116582) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Anwendung

• Anwendung: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.5-5 µg/mL ;

Zugehörige Ziele (menschlich)

MX1 Tbio Interferon-induced GTP-binding protein Mx1 (0 Activities)

Discover Target: MX1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MX1 Antibody ); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.