Nattokinase, CAS No.133876-92-3

CAS: 133876-92-3 Cat. No.: N646432 PubChem CID: 49867936

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, from Bacillus subtilis; ≥20000 FU/g enzyme powder

Bioactivity

≥20000 FU/g enzyme powder

Overview

  Nattokinase (CAS No.: 133876-92-3, EC 3.4.21.62), derived from Bacillus subtilis.

  It’s a food-grade alkaline serine protease refined and extracted through deep liquid fermentation.

  This product is an effective fibrinolytic enzyme, it can break down blood clots through directly hydrolyzing fibrin and fibrinolytic substrates, which can be used in the the study of cardiovascular diseases.

Product Feature

  Product appearance: Off-white to light brown powder, the colour may vary from batch to batch. Product odor: Slight odor of fermentation

  Standard enzyme activity: Not less than 20,000 FU/g

  Enzyme activity definition: Nattokinase reacts with fibrin to hydrolyze the fibrin peptide bond, and the hydrolysis reaction causes a change in the absorbance of the solution at 275 nm in the UV light. One unit of viability (1 FU) is the amount of enzyme consumed to make the absorbance value increase by 0.01 per increase in absorbance at 275 nm.

Application Principle and Method

  Fibrin is the main component of blood clots, which is a protein composed of polypeptide chains with strong stability and adhesion. Fibrinolytic enzyme can cleave the peptide bonds of fibrin, breaking it down into small molecules, thereby dissolving blood clots. The main function of nattokinase is to activate fibrinogen and convert it into fibrinolytic enzyme, thereby breaking down fibrin in blood clots and allowing them to dissolve.

  The thrombolytic effect of nattokinase has strong selectivity and specificity, and it can only act on fibrin in blood clots without affecting healthy blood vessel walls. Therefore, nattokinase is considered a safe and effective thrombolytic agent, which has been widely used in cardiovascular clinical treatment.

To prevent cardiovascular disease, the recommended dosage is 2000FU per day; For patients with cardiovascular and cerebrovascular diseases, it is recommended to take 4000-6000FU per day.

Specifications

Product Name

Nattokinase, CAS No.133876-92-3

Synonyms

Kinase, Natto fermentation | subtilisin | aprN

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, from Bacillus subtilis; ≥20000 FU/g enzyme powder

Biochemical and Physiological Mechanisms

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity.

Bioactivity

≥20000 FU/g enzyme powder

CAS

133876-92-3

Enzyme Commission Number

EC 3.4.21.62

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Bacillus subtilis; ≥20000 FU/g enzyme powder

Reconstitution

Reconstitute in reverse osmosis water.

Storage

Store at 2-8°C,Protected from light,Desiccated

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Store in the dark. Desiccated.

Unit definition

Nattokinase reacts with fibrin to hydrolyze the fibrin peptide bond, and the hydrolysis reaction causes a change in the absorbance of the solution at 275 nm in the UV light. One unit of viability (1 FU) is the amount of enzyme consumed to make the absorba

Associated Targets(Human)

PSMB5 Tclin Proteasome subunit beta type-5 (1 Activities)

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PSMB1 Tclin Proteasome subunit beta type-1 (1 Activities)

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F10 Tclin Coagulation factor X (9693 Activities)

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RPMI-8226 (44974 Activities)

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PSMB2 Tclin Proteasome Macropain subunit (1025 Activities)

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PSMB5 Tclin Proteasome Macropain subunit MB1 (2451 Activities)

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ARH-77 (115 Activities)

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U-266 (527 Activities)

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PSMB2 Tclin 20S proteasome (530 Activities)

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Associated Targets(non-human)

Hdac6 Histone deacetylase 6 (222 Activities)

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PRE7 Proteasome component C5 (37 Activities)

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rep Replicase polyprotein 1ab (378 Activities)

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clpP ATP-dependent Clp protease proteolytic subunit (245 Activities)

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Mechanisms of Action